MAB400-100

antibody from Novus Biologicals

Targeting: IL1A IL-1A, IL1, IL1-ALPHA, IL1F1

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB400-100

Provider

Novus Biologicals

Product name

Rat Monoclonal IL-1 alpha/IL-1F1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-1 alpha/IL-1F1 in ELISAs. In ELISAs, less than 1% cross-reactivity with recombinant rat IL-1 alpha/IL-1F1, recombinant porcine IL-1 alpha/IL-1F1, and recombinant human IL-1 alpha/IL-1F1 is observed.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-1 alpha/IL-1F1 and Neutralization by Mouse IL-1 alpha/IL-1F1 Antibody. Recombinant Mouse IL-1 alpha/IL-1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 alpha/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1 alpha/IL-1F1 Monoclonal Antibody (Catalog # MAB400). The ND₅₀ is typically 0.2-0.6 µg/mL in the presence of concanavalin A (1.25 µg/mL).