Antibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB200-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 alpha/IL-1F1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-1 alpha/IL-1F1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1 beta , -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha , -1 beta , -3, -4, -5, -6, -7, -9, or -13.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01583
- Isotype
- IgG
- Antibody clone number
- 4414
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling.
Redox control of the senescence regulator interleukin-1α and the secretory phenotype.
Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
Protection from RNA and DNA viruses by IL-32.
Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Ectopic expression of interleukin-1 receptor type II enhances cell migration through activation of the pre-interleukin 1alpha pathway.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Endogenous IL-32 controls cytokine and HIV-1 production.
Ultrasensitive flow-based immunoassays using single-molecule counting.
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway.
Henry CM, Martin SJ
Molecular cell 2017 Feb 16;65(4):715-729.e5
Molecular cell 2017 Feb 16;65(4):715-729.e5
Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling.
Beltrami-Moreira M, Vromman A, Sukhova GK, Folco EJ, Libby P
PloS one 2016;11(3):e0152474
PloS one 2016;11(3):e0152474
Redox control of the senescence regulator interleukin-1α and the secretory phenotype.
McCarthy DA, Clark RR, Bartling TR, Trebak M, Melendez JA
The Journal of biological chemistry 2013 Nov 8;288(45):32149-59
The Journal of biological chemistry 2013 Nov 8;288(45):32149-59
Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
Mucosal immunology 2012 Mar;5(2):140-9
Mucosal immunology 2012 Mar;5(2):140-9
Protection from RNA and DNA viruses by IL-32.
Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
International journal of cancer 2010 Jul 15;127(2):332-44
International journal of cancer 2010 Jul 15;127(2):332-44
Ectopic expression of interleukin-1 receptor type II enhances cell migration through activation of the pre-interleukin 1alpha pathway.
Chang SY, Su PF, Lee TC
Cytokine 2009 Jan;45(1):32-8
Cytokine 2009 Jan;45(1):32-8
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Gonzalez RM, Seurynck-Servoss SL, Crowley SA, Brown M, Omenn GS, Hayes DF, Zangar RC
Journal of proteome research 2008 Jun;7(6):2406-14
Journal of proteome research 2008 Jun;7(6):2406-14
Endogenous IL-32 controls cytokine and HIV-1 production.
Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Ultrasensitive flow-based immunoassays using single-molecule counting.
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clinical chemistry 2007 Nov;53(11):1990-5
Clinical chemistry 2007 Nov;53(11):1990-5
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Rao DA, Tracey KJ, Pober JS
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway.
Lee KY, Ho SC, Lin HC, Lin SM, Liu CY, Huang CD, Wang CH, Chung KF, Kuo HP
American journal of respiratory cell and molecular biology 2006 Oct;35(4):407-14
American journal of respiratory cell and molecular biology 2006 Oct;35(4):407-14
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 alpha/IL-1F1 in Human PBMCs. IL-1 alpha/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Human IL-1 alpha/IL-1F1 Monoclonal Antibody (Catalog # MAB200) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 alpha/IL-1F1 and Neutralization by Human IL-1 alpha/IL-1F1 Antibody. Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 alpha/IL-1F1 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1 alpha/IL-1F1 Monoclonal Antibody (Catalog # MAB200). The ND50 is typically 0.05-0.15 µg/mL in the presence of concanavalin A (1.25 µg/mL).