MAB400-100

antibody from R&D Systems

Targeting: IL1A IL-1A, IL1, IL1-ALPHA, IL1F1

ELISA (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB400-100

Provider

R&D Systems

Product name

Mouse IL-1 alpha/IL-1F1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-1 alpha/IL-1F1 in ELISAs. In ELISAs, less than 1% cross-reactivity with recombinant rat IL-1 alpha/IL-1F1, recombinant porcine IL-1 alpha/IL-1F1, and recombinant human IL-1 alpha/IL-1F1 is observed.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

P01582


Isotype

IgG

Antibody clone number

40508

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain.

Polykratis A, Martens A, Eren RO, Shirasaki Y, Yamagishi M, Yamaguchi Y, Uemura S, Miura M, Holzmann B, Kollias G, Armaka M, van Loo G, Pasparakis M
Nature cell biology 2019 Jun;21(6):731-742

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Alternatively activated macrophages determine repair of the infarcted adult murine heart.

Shiraishi M, Shintani Y, Shintani Y, Ishida H, Saba R, Yamaguchi A, Adachi H, Yashiro K, Suzuki K
The Journal of clinical investigation 2016 Jun 1;126(6):2151-66

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Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice.

Dankbar B, Fennen M, Brunert D, Hayer S, Frank S, Wehmeyer C, Beckmann D, Paruzel P, Bertrand J, Redlich K, Koers-Wunrau C, Stratis A, Korb-Pap A, Pap T
Nature medicine 2015 Sep;21(9):1085-90

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Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1α/IL-6 loop.

Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto KI, Simmons O, Toda S, Conway SJ, Hamasaki Y, Izuhara K
The Journal of investigative dermatology 2014 May;134(5):1295-1304

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Increased cytokine production in interleukin-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling.

Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
The Journal of biological chemistry 2009 Sep 18;284(38):25900-11

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Viral infection of the lungs through the eye.

Bitko V, Musiyenko A, Barik S
Journal of virology 2007 Jan;81(2):783-90

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-1 alpha/IL-1F1 and Neutralization by Mouse IL-1 alpha/IL-1F1 Antibody. Recombinant Mouse IL-1 alpha/IL-1F1 (Catalog # 400-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 alpha/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1 alpha/IL-1F1 Monoclonal Antibody (Catalog # MAB400). The ND₅₀ is typically 0.2-0.6 µg/mL in the presence of concanavalin A (1.25 µg/mL).