Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB2001-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 alpha/IL-1F1 Membrane Form Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-1 alpha/IL-1F1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-1 alpha , recombinant rat IL-1 alpha , recombinant cotton rat IL-1 alpha , recombinant human (rh) IL-1 beta or rhIL-18 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01583
- Isotype
- IgG
- Antibody clone number
- 3405
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-1 alpha/IL-1F1 and Neutralization by Human IL-1 alpha/IL-1F1 Antibody. Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 alpha/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1 alpha/IL-1F1 Membrane Form Monoclonal Antibody (Catalog # MAB2001). The ND50 is typically 5-20 µg/mL in the presence of concanavalin A (1.25 µg/mL).