Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP2-45400 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-1 alpha/IL-1F1 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Injury, dysbiosis, and filaggrin deficiency drive skin inflammation through keratinocyte IL-1α release.
Zaprinast diminished pain and enhanced opioid analgesia in a rat neuropathic pain model.
Archer NK, Jo JH, Lee SK, Kim D, Smith B, Ortines RV, Wang Y, Marchitto MC, Ravipati A, Cai SS, Dillen CA, Liu H, Miller RJ, Ashbaugh AG, Uppal AS, Oyoshi MK, Malhotra N, Hoff S, Garza LA, Kong HH, Segre JA, Geha RS, Miller LS
The Journal of allergy and clinical immunology 2019 Apr;143(4):1426-1443.e6
The Journal of allergy and clinical immunology 2019 Apr;143(4):1426-1443.e6
Zaprinast diminished pain and enhanced opioid analgesia in a rat neuropathic pain model.
Rojewska E, Piotrowska A, Jurga A, Makuch W, Mika J
European journal of pharmacology 2018 Nov 15;839:21-32
European journal of pharmacology 2018 Nov 15;839:21-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of HEK293T cells lysate (5 ug, left lane) and full length human recombinant protein of human IL-1 alpha (NP_000566) produced in Analysis of HEK293T cell (5 ug, right lane).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody. (1:200)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IL-1 alpha/IL-1F1 Antibody (2F8) [NBP2-45400] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)