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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB200R-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 alpha/IL-1F1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human IL-1 alpha/IL-1F1 in ELISAs and western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1 beta , -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha , -1 beta , -3, -4, -5, -6, -7, -9, or -13.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01583- Isotype
- IgG
- Antibody clone number
- 4414R
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 alpha/IL-1F1 in Human PBMCs. IL-1 alpha/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-1 alpha/IL-1F1 Recombinant Monoclonal Antibody (Catalog # MAB200R) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 alpha/IL-1F1 and Neutralization by Human IL-1 alpha/IL-1F1 Antibody. Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-1 alpha/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IL-1 alpha/IL-1F1 Recombinant Monoclonal Antibody (Catalog # MAB200R). The ND50 is typically 0.05-0.15 µg/mL.
