MA5-23694

antibody from Invitrogen Antibodies

Targeting: IL1A IL-1A, IL1, IL1-ALPHA, IL1F1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Recommended by provider)

Antibody Data

Product number

MA5-23694

Provider

Invitrogen Antibodies

Product name

IL-1 alpha Monoclonal Antibody (4414)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1 beta, -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha, -1 beta, -3, -4, -5, -6, -7, -9, or -13. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4414

Vial size

500 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of IL-1a/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL human IL-1a/IL-1F1 Monoclonal Antibody (Product # MA5-23694) for 3 hours at room temperature. Cells were stained with the 557-conjugated Anti-mouse IgG Secondary Antibody (re and counterstained with DAPI (blue).