H00003552-D01P

antibody from Abnova Corporation

Targeting: IL1A IL-1A, IL1, IL1-ALPHA, IL1F1

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003552-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003552-D01P, RRID:AB_1576274

Product name

IL1A purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human IL1A protein.

Antigen sequence

MAKVPDMFEDLKNCYSENEEDSSSIDHLSLNQKSF
YHVSYGPLHEGCMDQSVSLSISETSKTSKLTFKES
MVVVATNGKVLKKRRLSLSQSITDDDLEAIANDSE
EEIIKPRSAPFSFLSNVKYNFMRIIKYEFILNDAL
NQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSK
DDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEI
PKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIA
TKQDYWVCLAGGPPSITDFQILENQA


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of IL1A expression in transfected 293T cell line (H00003552-T01) by IL1A MaxPab polyclonal antibody.Lane 1: IL1A transfected lysate(30.60 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between IL1A and IL1R2. HeLa cells were stained with anti-IL1A rabbit purified polyclonal 1:1200 and anti-IL1R2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)