Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Proximity ligation assay [1]
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Validation data
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- Product number
- H00003552-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003552-D01P, RRID:AB_1576274
- Product name
- IL1A purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human IL1A protein.
- Antigen sequence
MAKVPDMFEDLKNCYSENEEDSSSIDHLSLNQKSF
YHVSYGPLHEGCMDQSVSLSISETSKTSKLTFKES
MVVVATNGKVLKKRRLSLSQSITDDDLEAIANDSE
EEIIKPRSAPFSFLSNVKYNFMRIIKYEFILNDAL
NQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSK
DDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEI
PKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIA
TKQDYWVCLAGGPPSITDFQILENQA- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of IL1A expression in transfected 293T cell line (H00003552-T01) by IL1A MaxPab polyclonal antibody.Lane 1: IL1A transfected lysate(30.60 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between IL1A and IL1R2. HeLa cells were stained with anti-IL1A rabbit purified polyclonal 1:1200 and anti-IL1R2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)