Antibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Other assay [2]
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- Product number
- 11-7118-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-1 alpha Monoclonal Antibody (364/3B3-14), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 364/3B3-14 antibody reacts with human interleukin-1alpha. Applications Reported:The 364/3B3-14 antibody has been reported for use as capture antibody in a human IL-1 alpha ELISA and for intracellular staining for flow cytometric analysis. Applications Tested: Has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 364/3B3-14
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references TIMP-1 is a novel ligand of Amyloid Precursor Protein and triggers a proinflammatory phenotype in human monocytes.
DOT1L modulates the senescence-associated secretory phenotype through epigenetic regulation of IL1A.
Effects of chlorpyrifos and trichloropyridinol on HEK 293 human embryonic kidney cells.
Eckfeld C, Schoeps B, Häußler D, Frädrich J, Bayerl F, Böttcher JP, Knolle P, Heisz S, Prokopchuk O, Hauner H, Munkhbaatar E, Demir IE, Hermann CD, Krüger A
The Journal of cell biology 2023 Feb 6;222(2)
The Journal of cell biology 2023 Feb 6;222(2)
DOT1L modulates the senescence-associated secretory phenotype through epigenetic regulation of IL1A.
Leon KE, Buj R, Lesko E, Dahl ES, Chen CW, Tangudu NK, Imamura-Kawasawa Y, Kossenkov AV, Hobbs RP, Aird KM
The Journal of cell biology 2021 Aug 2;220(8)
The Journal of cell biology 2021 Aug 2;220(8)
Effects of chlorpyrifos and trichloropyridinol on HEK 293 human embryonic kidney cells.
Van Emon JM, Pan P, van Breukelen F
Chemosphere 2018 Jan;191:537-547
Chemosphere 2018 Jan;191:537-547
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. DOT1L is necessary for H3K79me2/3 at the IL1A locus and SASP expression but dispensable for other senescence phenotypes. IMR90 cells were infected with retrovirus-expressing HRAS G12V (RAS) or empty vector control with or without shRNA to human DOT1L (shDOT1L) or an shGFP control. Details on time points are in Fig. S1 A . (A) Immunoblot analysis of total cell lysates (TCL) and chromatin fractions of the indicated proteins. beta-Actin was used as a loading control for TCL. Histone H3 was used as a loading control for chromatin fractions. One of three independent experimental replicates is shown. (B) H3K79me2 and DOT1L binding to the IL1A promoter region and H3K79me2 at the promoters of IL6 , CXCL8 , and IL1B was determined by ChIP-qPCR and normalized to total histone H3 binding at the same site. One of three independent experimental replicates is shown. Data represent mean +- SD ( n = 3). *, P < 0.05 by one-way ANOVA with Tukey's multiple comparisons. (C) H3K79me3 and DOT1L binding to the IL1A gene body and H3K79me3 at the gene bodies of IL6 , CXCL8 , and IL1B was determined by ChIP-qPCR and normalized to total histone H3 binding at the same site. One of three independent experimental replicates is shown. Data represent mean +- SD ( n = 3). *, P < 0.05 by one-way ANOVA with Tukey's multiple comparisons. (D) IL1A mRNA expression was determined by RT-qPCR. One of five independent experimental replicates is shown. Data represent mean +- SD ( n = 3). *, P < 0.01 by one-w
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. DOT1L overexpression (OE) increases H3K79me2/3 at the IL1A locus and is sufficient for SASP gene expression but does not affect other senescence phenotypes. IMR90 cells were infected with retrovirus-expressing human DOT1L or empty vector control. In some experiments, as a positive control, IMR90 cells were infected with retrovirus-expressing HRAS G12V (RAS) or empty vector control. (A) DOT1L mRNA expression. One of three independent experimental replicates is shown. Data represent mean +- SD ( n = 3). *, P < 0.001 by Student's t test. (B) DOT1L immunoblot analysis. Vinculin was used as a loading control. One of five independent experimental replicates is shown. (C) H3K79me2 and H3K79me3 immunoblot analysis on chromatin fractions. Total histone H3 was used as loading control. One of three independent experimental replicates is shown. (D) H3K79me2 and DOT1L binding to the IL1A promoter region and H3K79me2 at the promoters of IL6 , CXCL8 , and IL1B was determined by ChIP-qPCR and normalized to total histone H3 binding at the same site. One of three independent experimental replicates is shown. Data represent mean +- SD ( n = 3). *, P < 0.05 by Student's t test. (E) H3K79me3 and DOT1L binding to the IL1A gene body and H3K79me3 at the gene bodies of IL6 , CXCL8 , and IL1B was determined by ChIP-qPCR and normalized to total histone H3 binding at the same site. One of three independent experimental replicates is shown. Data represent mean +- SD ( n = 3). *, P < 0.05 by Stu
- Conjugate
- Green dye