Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-85366 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-1 alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 1.09 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-1 alpha in A) HepG2 cells using IL-1 alpha polyclonal antibody (Product # PA5-85366) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 12% SDS PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IL-1 alpha Polyclonal Antibody (Product # PA5-85366). Sample (30 µg of whole cell lysate). Lane A: HepG2. 12% SDS PAGE. IL-1 alpha Polyclonal Antibody (Product # PA5-85366) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL-1 alpha Polyclonal Antibody detects IL1 alpha protein at cytoplasm by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL1 alpha protein stained by IL-1 alpha Polyclonal Antibody (Product # PA5-85366) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IL1 alpha (Product # PA5-85366) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL-1 alpha Polyclonal Antibody detects IL1 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. IL1 alpha stained by IL-1 alpha Polyclonal Antibody (Product # PA5-85366) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
