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- Antibody Data
- Antigen structure
- References [2]
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- Other assay [1]
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- Product number
- M421A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-1 alpha Monoclonal Antibody (ILA9-H18)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M421A targets IL-1 alpha in ELISA applications and shows reactivity with Human samples. The M421A immunogen is recombinant human IL-1 alpha. M421A detects IL-1 alpha which has a predicted molecular weight of approximately 18 kDa. The M421A IL1a antibody (clone ILA9-H18) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M420AB (biotinylated conjugate of clone ILA8-H12). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. Antibody M421A (clone ILA9-H18) and biotinylated antibody M420AB (clone ILA8-H12) have successfully been used in combination with recombinant IL1 alpha protein SIL1A in ELISA applications. This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- ILA9-H18
- Vial size
- 500 µg
- Concentration
- 1.0 mg/mL
- Storage
- -20°C
Submitted references Bioactive interleukin-1alpha is cytolytically released from Candida albicans-infected oral epithelial cells.
Prostaglandin E2 induces the final maturation of IL-12-deficient CD1a+CD83+ dendritic cells: the levels of IL-12 are determined during the final dendritic cell maturation and are resistant to further modulation.
Dongari-Bagtzoglou A, Kashleva H, Villar CC
Medical mycology 2004 Dec;42(6):531-41
Medical mycology 2004 Dec;42(6):531-41
Prostaglandin E2 induces the final maturation of IL-12-deficient CD1a+CD83+ dendritic cells: the levels of IL-12 are determined during the final dendritic cell maturation and are resistant to further modulation.
Kaliński P, Schuitemaker JH, Hilkens CM, Kapsenberg ML
Journal of immunology (Baltimore, Md. : 1950) 1998 Sep 15;161(6):2804-9
Journal of immunology (Baltimore, Md. : 1950) 1998 Sep 15;161(6):2804-9
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA analysis of human IL-1 alpha was performed using a Human IL-1 alpha Colorimetric ELISA Kit (Product # EH2IL1A) by loading 50 µL per well of Human IL-1 alpha Recombinant Protein (Product # SIL1A) in dodecuplicate at 400, 160, 64, 25.6, 10.25 and 0 pg/mL across a 3 µg/mL mouse anti-human IL-1 alpha (Product # M421A) pre-coated plate and incubating for 1 hour at room temperature. The plate was washed, then incubated with 50 µL per well of a biotinylated-mouse anti-human IL-1 alpha (Product # M420AB) in dodecuplicate at 0.15 µg/mL for 1 hour at room temperature. The plate was washed and incubated with 100 µL per well of Ultra Streptavidin-HRP (Product # N504) in all test wells at 1:10 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB Substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
