Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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Comment
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- Product number
- PA5-25921 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-1 alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The epithelial danger signal IL-1α is a potent activator of fibroblasts and reactivator of intestinal inflammation.
Scarpa M, Kessler S, Sadler T, West G, Homer C, McDonald C, de la Motte C, Fiocchi C, Stylianou E
The American journal of pathology 2015 Jun;185(6):1624-37
The American journal of pathology 2015 Jun;185(6):1624-37
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using an IL1A polyclonal antibody (Product # PA5-25921) in HepG2 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of NCI-H460 cells using an IL1A polyclonal antibody (Product # PA5-25921) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL