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- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-200-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 alpha/IL-1F1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-1 alpha/IL-1F1 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse (rm) IL-1 alpha and recombinant rat IL-1 alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q53QF9- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis.
Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients.
P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
The response of human epithelial cells to TNF involves an inducible autocrine cascade.
Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1).
Fas and Fas-associated death domain protein regulate monocyte chemoattractant protein-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of interleukin-1alpha.
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Qiang L, Sample A, Shea CR, Soltani K, Macleod KF, He YY
Autophagy 2017;13(12):2086-2103
Autophagy 2017;13(12):2086-2103
Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients.
Savill SA, Leitch HF, Harvey JN, Thomas TH
PloS one 2016;11(9):e0162908
PloS one 2016;11(9):e0162908
P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
British journal of pharmacology 2007 May;151(1):115-27
British journal of pharmacology 2007 May;151(1):115-27
The response of human epithelial cells to TNF involves an inducible autocrine cascade.
Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK
Cell 2006 Mar 24;124(6):1225-39
Cell 2006 Mar 24;124(6):1225-39
Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1).
Palmer G, Trolliet S, Talabot-Ayer D, Mézin F, Magne D, Gabay C
Cytokine 2005 Jul 21;31(2):153-60
Cytokine 2005 Jul 21;31(2):153-60
Fas and Fas-associated death domain protein regulate monocyte chemoattractant protein-1 expression by human smooth muscle cells through caspase- and calpain-dependent release of interleukin-1alpha.
Schaub FJ, Liles WC, Ferri N, Sayson K, Seifert RA, Bowen-Pope DF
Circulation research 2003 Sep 19;93(6):515-22
Circulation research 2003 Sep 19;93(6):515-22
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Sengupta J, Dhawan L, Ghosh D
Cytokine 2003 Dec 21;24(6):277-85
Cytokine 2003 Dec 21;24(6):277-85
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Sengupta J, Dhawan L, Ghosh D
Cytokine 2003 Dec 21;24(6):277-85
Cytokine 2003 Dec 21;24(6):277-85
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 alpha/IL-1F1 in Human PBMCs. IL-1 alpha/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS using Goat Anti-Human IL-1 alpha/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 alpha/IL-1F1 and Neutralization by Human IL-1 alpha/IL-1F1 Antibody. Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 alpha/IL-1F1 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 alpha/IL-1F1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-200-NA). The ND50 is typically 0.75-4.5 ng/mL.
