Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BAF200 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 alpha/IL-1F1 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-1 alpha/IL-1F1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse IL-1 alpha , recombinant porcine IL-1 alpha , recombinant rat IL-1 alpha , recombinant human (rh) IL-1 sRI, and rhIL-1 sRII is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
Q53QF9- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Protection from RNA and DNA viruses by IL-32.
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Endogenous IL-32 controls cytokine and HIV-1 production.
Endogenous IL-32 controls cytokine and HIV-1 production.
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Ultrasensitive flow-based immunoassays using single-molecule counting.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.
Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 1;183(9):5799-806
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Gonzalez RM, Seurynck-Servoss SL, Crowley SA, Brown M, Omenn GS, Hayes DF, Zangar RC
Journal of proteome research 2008 Jun;7(6):2406-14
Journal of proteome research 2008 Jun;7(6):2406-14
Endogenous IL-32 controls cytokine and HIV-1 production.
Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Endogenous IL-32 controls cytokine and HIV-1 production.
Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Pfleger C, Schloot N, ter Veld F
Journal of immunological methods 2008 Jan 1;329(1-2):214-8
Journal of immunological methods 2008 Jan 1;329(1-2):214-8
Ultrasensitive flow-based immunoassays using single-molecule counting.
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clinical chemistry 2007 Nov;53(11):1990-5
Clinical chemistry 2007 Nov;53(11):1990-5
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.
Chen DS, Soen Y, Stuge TB, Lee PP, Weber JS, Brown PO, Davis MM
PLoS medicine 2005 Oct;2(10):e265
PLoS medicine 2005 Oct;2(10):e265
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 alpha/IL-1F1 in Human PBMCs. IL-1 alpha/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Human IL-1 alpha/IL-1F1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF200) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 alpha/IL-1F1 in Human PBMCs. IL-1 alpha/IL-1F1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Human IL-1 alpha/IL-1F1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF200) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
