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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC200F - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 alpha/IL-1F1 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-1 alpha/IL-1F1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1 beta , -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha , -1 beta , -3, -4, -5, -6, -7, -9, or -13.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P01583- Isotype
- IgG
- Antibody clone number
- 4414
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence.
Ong SM, Hadadi E, Dang TM, Yeap WH, Tan CT, Ng TP, Larbi A, Wong SC
Cell death & disease 2018 Feb 15;9(3):266
Cell death & disease 2018 Feb 15;9(3):266
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-1 alpha/IL-1F1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes (A) treated with LPS were stained with Mouse Anti-Human IL-1 alpha/IL-1F1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC200F) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC002F). (B) Inhibition of Mouse Anti-Human IL-1 alpha/IL-1F1 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC200F) staining by the addition of excess Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
