Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 16-1249-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD124 Monoclonal Antibody (X2/45-12), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The X2/45-12 monoclonal antibody reacts with the human IL-4 receptor alpha chain. IL-4 receptor alpha chain (CD124) is crucial for the binding and signal transduction of IL-4. Both IL-4 and IL-13 use the IL-4 receptor alpha chain as their receptor component and activate many common signal pathways. Applications Reported: X2/45-12 has been reported for use in flow cytometric analysis. X2/45-12 has also been reported in neutralization of human IL-4 function. Applications Tested: The X2/45-12 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- X2/45-12
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Interleukin-13 overexpression by tax transactivation: a potential autocrine stimulus in human T-cell leukemia virus-infected lymphocytes.
Design of human interleukin-4 antagonists inhibiting interleukin-4-dependent and interleukin-13-dependent responses in T-cells and B-cells with high efficiency.
Wäldele K, Schneider G, Ruckes T, Grassmann R
Journal of virology 2004 Jun;78(12):6081-90
Journal of virology 2004 Jun;78(12):6081-90
Design of human interleukin-4 antagonists inhibiting interleukin-4-dependent and interleukin-13-dependent responses in T-cells and B-cells with high efficiency.
Tony HP, Shen BJ, Reusch P, Sebald W
European journal of biochemistry 1994 Oct 15;225(2):659-65
European journal of biochemistry 1994 Oct 15;225(2):659-65
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Surface staining of normal human peripheral blood cells with Anti-Human CD124 Purified followed by Anti-Mouse IgG Biotin and Streptavidin PE (Product # 12-4317-87). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
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