Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 355003 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#355003, RRID:AB_11218807
- Product name
- PE anti-human CD124 (IL-4R)
- Antibody type
- Monoclonal
- Antigen
- Recombinant human IL-4Rα Fc chimera
- Description
- CD124, also known as the alpha subunit of IL-4R, is a 140 kD transmembrane glycoprotein. It associates with either the common γ-chain (CD132) to form the type I IL-4R complex, which specifically binds IL-4, or with IL-13Ra1 to form the type II IL-4R heterodimeric complex, which binds and transduces signals from either IL-4 or IL-13. A truncated form of IL-4Rα exists in the soluble form in biological fluids. CD124 is expressed on human B and T cells as well as a variety of other hematopoietic and non-hematopoietic cells and cell lines. In B cells, CD124 can bind with IL-4 and IL-13 to regulate IgE antibody production. In T cells, the type I IL-4R (IL-4R/gC) is mostly responsible for Th2 cell expansion by mediating IL-4-dependent activation of the transcription factors in hematopoietic cells. The type II IL-4R (IL-4R/IL-13Ra1) is the main route for non-hematopoietic cell responses to IL-4 or IL-13.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 25 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human peripheral blood lymphocytes were stained with CD19 APC and CD124 (clone G077F6) PE (filled histogram) or mouse IgG2a, κ isotype control PE (open histogram). The data was analyzed by gating on CD19-positive cells.
- Conjugate
- Yellow dye