500-P325-1MG

antibody from Invitrogen Antibodies
Targeting: IL4R CD124
Provider product page for 500-P325-1MG
Western blot
ELISA

Antibody data

Product number
500-P325-1MG - Provider product page
Provider
Invitrogen Antibodies
Product name
IL4R (soluble) Polyclonal Antibody, PeproTech®
Antibody type
Polyclonal
Antigen
Recombinant full-length protein
Description
AA Sequence of recombinant protein: GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-4 Receptor alpha. Anti-Human sIL-4 Receptor alpha-specific antibody was purified by affinity chromatography employing an immobilized Human sIL-4 Receptor alpha matrix. Sandwich ELISA: To detect Human sIL-4 Receptor alpha by sandwich ELISA (using 100 µL/well), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sIL-4 Receptor alpha (500-P325BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human sIL-4 Receptor alpha. Western Blot: To detect Human sIL-4 Receptor alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human sIL-4 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity
Human
Host
Rabbit
Vial size
1 mg
Concentration
0.1-1.0 mg/mL
Storage
-20°C
IL4R protein structure - 500-P325-1MG shown in red.