16389-1-AP

antibody from Proteintech Group

Targeting: XRCC5 KARP-1, KU80, Ku86, KUB2

Provider product page for 16389-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [45]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 16389-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16389-1-AP, RRID:AB_2257509
Product name: XRCC5/Ku80 antibody
Antibody type: Polyclonal
Description: KD/KO validated XRCC5/Ku80 antibody (Cat. #16389-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

XRCC5 protein structure - 16389-1-AP shown in red.

Submitted references O-GlcNAcylation of NONO mediates alternative splicing of SETMAR and facilitates NHEJ repair.

Li M, Tian H, Zhou Z, Jiang Y, Guo X, Qin W, Zhang H, Jiao Y, Guo S, Wu C
Genome biology 2026 Jan 14;27(1):26

FOXN3 integrates the KU70/KU80/SREBP-1 complex to regulate lipid metabolism in non-alcoholic fatty liver disease.

Du J, Jin L, Wang S, Hu X, Yu J, Yu J, Huo Q, Wu N, Liu X, Yang Y, Zhang Y, Huang S, Zhou J, Zheng SG, Zheng C, Zhu X
Nucleic acids research 2026 Feb 24;54(5)

Invivo systematic detection of the outcomes of CRISPR-Cas9-mediated DNA repair in skeletal muscle stem cells.

He L, Fu Y, Wang Z, Zhou Q, Sun H, Wang H
Molecular therapy : the journal of the American Society of Gene Therapy 2026 Apr 20;

Targeting ESR1 restores SQSTM1-dependent autophagy and sensitizes ER-positive breast cancer to oxidative and radiation stress.

Yang YF, He ZJ, Kuo HH, Lin YY, Kim CH, Cai HY, Chen CL, Hsiao M, Chen YC, Chang PM, Chang YC
Cell death discovery 2025 Oct 7;11(1):451

APOL2 Stabilizes Ku80 to Confer NHEJ-Mediated Radioresistance in Gastric Cancer.

Zu D, Bao Q, He H, Zhong Y, Deng M, Hu Y, Zhang C, Liang C, Huang Y, Liu H, Li X, He Y, Luo G, Wu W, Guan F, Xu S, Liu M, Bacolla A, Jing J, Du Y, Tainer JA, Shi Y, Ye Z, Cheng X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Nov;12(44):e06294

KAT2A-driven succinylation of SRSF11 enforces spliceosome-mediated RAD52 splicing to promote homologous recombination and radioresistance in hepatocellular carcinoma.

Wu J, Yuan J, Liu Z, Zhou Y, Zhang B, Xu Y, Zeng Q, Wu Z, Kong L, Wang J, Zhang B, Yang J, Lv T, Shi Y, Yang J
Signal transduction and targeted therapy 2025 Nov 7;10(1):364

CNOT7 facilitates radiation resistance in colorectal cancer through TRIM21/XRCC6-mediated non-homologous end joining repair.

Li Y, Cui L, Wang S, Zhao Q, Du F, Du S, Yu C, Xia M, Zhao S, Luan T, Liu Y, Cui B
Cell death & disease 2025 Nov 17;16(1):833

Elevated nonhomologous end-joining by AATF enables efficient DNA damage repair and therapeutic resistance in glioblastoma.

Mi L, Cai Y, Qi J, Chen L, Li Y, Zhang S, Ran H, Qi Q, Zhang C, Wu H, Cao S, Huang H, Xiao D, Wang X, Li B, Xie J, Li F, Han Q, Wu Q, Li T, Li A, Rich JN, Zhou T, Man J
Nature communications 2025 May 28;16(1):4941

Nuclear VPS35 attenuates NHEJ repair by sequestering Ku protein.

Zhang L, Nie Y, Tang T, Lu Y, Li W, Hong X, Li Q, Zheng A, Li Y, Zhou J, Fan L, Wang T, Deng Z
Molecular medicine (Cambridge, Mass.) 2025 Jun 9;31(1):222

Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.

He J, Lai T, Zhou Z, Yang H, Lei Z, Zhou L, Li N, He Y, Zeng S, Munai E, Tan Y, Wang M, Zhang Y, Zhou W, Wu Y
Scientific reports 2025 Jul 9;15(1):24651

Moesin silencing enhances the radiosensitivity of glioblastoma by inhibiting DNA damage repair via CD44/AKT1 signaling pathway.

Ding X, Wang Y, Shao Z, Wen X, Wang B, Liu G, Xu C, Chai L, Qiu H, Zhang L
Discover oncology 2025 Jul 31;16(1):1451

Chromatin Perturbation Promotes Susceptibility to Hypomethylating Agents.

Schneider C, Alexe G, Merickel LA, Meyer A, Swift ML, Serafim RB, Basanthakumar AT, Taillon A, Salhotra S, Boniolo F, Lambo S, Rodriguez Y, Häupl B, George RE, Root DE, Oellerich T, Hovestadt V, Chowdhury D, Stegmaier K
bioRxiv : the preprint server for biology 2025 Jul 31;

LINC01235 Promotes Clonal Evolution through DNA Replication Licensing-Induced Chromosomal Instability in Breast Cancer.

Zhang Q, Wang X, Shao Z, Zhang Y, Zhang L, Chen M, Zhou X, Zhu H, Zhou Y, Lu X, Li P, Chi W, Li L, Shao ZM, Huang S, Xue J, Chi Y, Wu J, Xiu B
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Apr;12(14):e2413527

LncRNA FTX promotes colorectal cancer radioresistance through disturbing redox balance and inhibiting ferroptosis via miR-625-5p/SCL7A11 axis.

Dai Q, Qu TY, Yang JL, Leng J, Fang L, Zhu QQ, Wu KB, Wu J, Ma JJ, Yu HF
World journal of gastroenterology 2025 Apr 28;31(16):104305

Polymorphism in the Hsa-miR-4274 seed region influences the expression of PEX5 and enhances radiotherapy resistance in colorectal cancer.

Lu Q, Ren N, Chen H, Zhang S, Yan R, Li M, Zheng L, Tan W, Lin D
Frontiers of medicine 2024 Oct;18(5):921-937

CircUGGT2 facilitates progression and cisplatin resistance of bladder cancer through nonhomologous end-joining pathway.

Lyu F, Huang S, Yan Z, He Q, Liu C, Cheng L, Cong Y, Chen K, Song Y, Xing Y
Cellular signalling 2024 Jul;119:111164

FAM21 interacts with Ku to promote the localization of WASH to DNA double strand break sites.

Wang T, Zheng AX, Li P, Tang T, Zhang LP, Hong Y, Hong X, Deng ZH
DNA repair 2024 Jan;133:103603

PICH Activates Cyclin A1 Transcription to Drive S-Phase Progression and Chemoresistance in Gastric Cancer.

Ye H, Shi W, Yang J, Wang L, Jiang X, Zhao H, Qin L, Qin J, Li L, Cai W, Guan J, Yang H, Zhou H, Yu Z, Sun H, Jiao Z
Cancer research 2023 Nov 15;83(22):3767-3782

NAD(+) rescues aging-induced blood-brain barrier damage via the CX43-PARP1 axis.

Zhan R, Meng X, Tian D, Xu J, Cui H, Yang J, Xu Y, Shi M, Xue J, Yu W, Hu G, Li K, Ge X, Zhang Q, Zhao M, Du J, Guo X, Xu W, Gao Y, Yao C, Chen F, Chen Y, Shan W, Zhu Y, Ji L, Pan B, Yu Y, Li W, Zhao X, He Q, Liu X, Huang Y, Liao S, Zhou B, Chui D, Chen YE, Sun Z, Dong E, Wang Y, Zheng L
Neuron 2023 Nov 15;111(22):3634-3649.e7

LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer.

Dey G, Bharti R, Braley C, Alluri R, Esakov E, Crean-Tate K, McCrae K, Joehlin-Price A, Rose PG, Lathia J, Gong Z, Reizes O
Journal of ovarian research 2023 Jun 27;16(1):122

Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy.

Wei Y, Xiao G, Xu H, Sun X, Shi Y, Wang F, Kang J, Peng J, Zhou F
The FEBS journal 2023 Jul;290(14):3629-3645

Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer.

Chen M, Zhao E, Li M, Xu M, Hao S, Gao Y, Wu X, Li X, Yu Y, Yu Z, Yin Y
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 Jul 25;116:154876

Screening and characterization of myositis-related autoantibodies in COVID-19 patients.

Teo KF, Chen DY, Hsu JT, Lai YH, Chang CK, Hsueh PR, Lan JL, Hsu JL
Clinical and translational science 2023 Jan;16(1):140-150

ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway.

Wen X, Chen S, Chen X, Qiu H, Wang W, Zhang N, Liu W, Wang T, Ding X, Zhang L
Frontiers in oncology 2022;12:887068

circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells.

Li Q, Yao H, Wang Y, Wu Y, Thorne RF, Zhu Y, Wu M, Liu L
Cell reports 2022 Nov 22;41(8):111707

WASH interacts with Ku to regulate DNA double-stranded break repair.

Wang T, Du XH, Hong Y, Hong X, Fan L, Zhou JW, Sun H, Ge J, Billadeau DD, Deng ZH
iScience 2022 Jan 21;25(1):103676

Acquired temozolomide resistance in MGMT(low) gliomas is associated with regulation of homologous recombination repair by ROCK2.

Zhang X, Li T, Yang M, Du Q, Wang R, Fu B, Tan Y, Cao M, Chen Y, Wang Q, Hu R
Cell death & disease 2022 Feb 10;13(2):138

Proteomic Analysis of Vero Cells Infected with Pseudorabies Virus.

Yang X, Xu S, Chen D, Jiang R, Kang H, Ge X, Zhou L, Han J, Zhang Y, Guo X, Yang H
Viruses 2022 Apr 4;14(4)

Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway.

Wang L, Huang Y, Liu C, Guo M, Ma Z, He J, Wang A, Sun X, Liu Z
Journal of Cancer 2021;12(3):860-873

Epithelial cell transforming factor ECT2 is an important regulator of DNA double-strand break repair and genome stability.

Cao C, Han P, Liu L, Tang Y, Tian S, Zhang K, Shi L, Liu Z, Zhuo D, Ge W, Gong W
The Journal of biological chemistry 2021 Sep;297(3):101036

Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca(2+)](m)-PDP1-PDH-histone acetylation retrograde signaling.

Shi Y, Wang Y, Jiang H, Sun X, Xu H, Wei X, Wei Y, Xiao G, Song Z, Zhou F
Cell death & disease 2021 Sep 6;12(9):837

Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shortening.

Tichy ED, Ma N, Sidibe D, Loro E, Kocan J, Chen DZ, Khurana TS, Hasty P, Mourkioti F
Cell reports 2021 May 11;35(6):109098

Elevated XRCC5 expression level can promote temozolomide resistance and predict poor prognosis in glioblastoma.

Lee IN, Yang JT, Huang C, Huang HC, Wu YP, Chen JC
Oncology letters 2021 Jun;21(6):443

Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer.

Li S, Sun W, Luo Y, Gao Y, Jiang X, Yuan C, Han L, Cao K, Gong Y, Xie C
Journal of materials chemistry. B 2021 Jun 16;9(23):4643-4653

OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair.

Cui Y, Xie R, Zhang X, Liu Y, Hu Y, Li Y, Liu X, Yu X, Wu C
Cell death & disease 2021 Jun 16;12(7):622

LncRNA linc00312 suppresses radiotherapy resistance by targeting DNA-PKcs and impairing DNA damage repair in nasopharyngeal carcinoma.

Guo Z, Wang YH, Xu H, Yuan CS, Zhou HH, Huang WH, Wang H, Zhang W
Cell death & disease 2021 Jan 4;12(1):69

Cytoplasmic DNA sensing by KU complex in aged CD4(+) T cell potentiates T cell activation and aging-related autoimmune inflammation.

Wang Y, Fu Z, Li X, Liang Y, Pei S, Hao S, Zhu Q, Yu T, Pei Y, Yuan J, Ye J, Fu J, Xu J, Hong J, Yang R, Hou H, Huang X, Peng C, Zheng M, Xiao Y
Immunity 2021 Apr 13;54(4):632-647.e9

DNA methylation‑regulated miR‑155‑5p depresses sensitivity of esophageal carcinoma cells to radiation and multiple chemotherapeutic drugs via suppression of MAP3K10.

Luo W, Zhang H, Liang X, Xia R, Deng H, Yi Q, Lv L, Qian L
Oncology reports 2020 May;43(5):1692-1704

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.

Li G, Zhang X, Wang H, Liu D, Li Z, Wu Z, Yang H
The international journal of biochemistry & cell biology 2020 Aug;125:105790

Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes.

Martin JH, Bromfield EG, Aitken RJ, Lord T, Nixon B
Scientific reports 2018 Jun 26;8(1):9685

Flavone protects HBE cells from DNA double-strand breaks caused by PM2.5.

Ren X, Tang Y, Sun J, Feng J, Chen L, Chen H, Zeng S, Chen C, Li X, Zhu H, Zeng Z
Human cell 2018 Apr;31(2):116-126

Downregulation of Mitochondrial Single Stranded DNA Binding Protein (SSBP1) Induces Mitochondrial Dysfunction and Increases the Radiosensitivity in Non-Small Cell Lung Cancer Cells.

Wang Y, Hu L, Zhang X, Zhao H, Xu H, Wei Y, Jiang H, Xie C, Zhou Y, Zhou F
Journal of Cancer 2017;8(8):1400-1409

UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.

Hu L, Li X, Liu Q, Xu J, Ge H, Wang Z, Wang H, Wang Z, Shi C, Xu X, Huang J, Lin Z, Pieper RO, Weng C
Oncogene 2017 Feb 23;36(8):1145-1156

Sensitization of tamoxifen-resistant breast cancer cells by Z-ligustilide through inhibiting autophagy and accumulating DNA damages.

Qi H, Jiang Z, Wang C, Yang Y, Li L, He H, Yu Z
Oncotarget 2017 Apr 25;8(17):29300-29317

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Dong H, Shi Q, Song X, Fu J, Hu L, Xu D, Su C, Xia X, Song E, Song Y
Toxicology and applied pharmacology 2015 Jul 1;286(1):10-6

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 16389-1-AP(XRCC5 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using XRCC5 antibody 16389-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 16389-1-AP(XRCC5 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 16389-1-AP(XRCC5 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue