Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-508 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-508, RRID:AB_2241915
- Product name
- Rabbit Polyclonal Ku80/XRCC5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Reacts with residues 419-440 [LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse, rat and hamster.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Significant correlation of species longevity with DNA double strand break recognition but not with telomere length.
Lorenzini A, Johnson FB, Oliver A, Tresini M, Smith JS, Hdeib M, Sell C, Cristofalo VJ, Stamato TD
Mechanisms of ageing and development 2009 Nov-Dec;130(11-12):784-92
Mechanisms of ageing and development 2009 Nov-Dec;130(11-12):784-92
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ku80/XRCC5 Antibody [NB100-508] - Analysis of Ku80 using NB100-508 at 1:500 dilution on 50 ug of total lysates of A431 (lane 1) and AR42J (lane 2) cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Ku80/XRCC5 Antibody [NB100-508] - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-508. A Ku80 deficient cell lysate, XRS, was used as a negative control.
