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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunoprecipitation [1]
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- Product number
- NB100-503 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-503, RRID:AB_2218753
- Product name
- Rabbit Polyclonal Ku80/XRCC5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Reacts with residues 323-338 [FSKVDEEQMKYKSEGK] of the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse and rat.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references BCL2-overexpressing prostate cancer cells rely on PARP1-dependent end-joining and are sensitive to combined PARP inhibitor and radiation therapy.
Oing C, Tennstedt P, Simon R, Volquardsen J, Borgmann K, Bokemeyer C, Petersen C, Dikomey E, Rothkamm K, Mansour WY
Cancer letters 2018 Jun 1;423:60-70
Cancer letters 2018 Jun 1;423:60-70
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: Ku80/XRCC5 Antibody [NB100-503] - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-503. A Ku80 deficient cell lysate, XRS, was used as a negative control.
