Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C500039 - Provider product page
- Provider
- LSBio
- Product name
- XRCC5 / Ku80 Antibody LS-C500039
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Positive WB detected in:HepG2 whole cell lysate,LO2 whole cell lysate,Mouse liver tissue;All lanes:XRCC5 antibody at 3.5?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 83 KDa;Observed band size: 83 KDa;
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot Positive WB detected in: LO2 whole cell lysate All lanes: XRCC5 antibody at 3.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 83 kDa Observed band size: 83 kDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating XRCC5 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of XRCC5 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: XRCC5 Antibody (8µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating XRCC5 in HepG2 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of XRCC5 Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: XRCC5 Antibody (8µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human melanoma cancer using XRCC5 Antibody at dilution of 1:100
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using XRCC5 Antibody at dilution of 1:100