MA1-10390

antibody from Invitrogen Antibodies

Targeting: XRCC5 KARP-1, KU80, Ku86, KUB2

Immunoprecipitation

Antibody data

Submit

Validation data

XRCC5 protein structure - MA1-10390 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence staining of HeLa human cervix carcinoma cell line using purified anti-Ku Antigen (MEM-54) (detection by Goat anti-mouse IgG2a Alexa Fluor® 488; green). Actin (red) was stained with Phalloidin-TRITC, nuclei with DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry staining of HeLa human cervix carcinoma cell line using purified anti-Ku Antigen (MEM-54) Monoclonal antibody (Product # MA1-10390) (detection by Goat anti-mouse IgG2a Alexa Fluor® 488; green). Actin (red) was stained with Phalloidin-TRITC, nuclei with DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Ku80 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Ku80 Monoclonal Antibody (MEM-54) (Product # MA1-10390) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing staining in nucleus. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Ku80 was achieved by transfecting HeLa cells with Ku80 specific siRNA (Silencer® select Product # s14952, s14953). Immunofluorescence analysis was performed on untransfected HeLa cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with Ku80 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with Ku80 Monoclonal Antibody (MEM-54) (Product # MA1-10390, 1:200 dilution) followed by Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766, (1:2000 dilution). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (Red) staining. Reduction of specific signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to Ku80 (Green). The Images were captured at 60X magnification.