Explore
Validate
Learn
Western blot
Immunocytochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [47]
- Comments [0]
- Validations
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-12930 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Ku80 Monoclonal Antibody (111), Biotin
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-12933 targets Ku (p80) in FACS, ICC/IF, IP, and WB applications and shows reactivity with Human, Mouse and Non-human primate samples. This antibody does not cross-react with mouse in immunofluorescent applications, but does react with mouse in FACS applications. The MA5-12933 immunogen is human B cell nuclei from plasmacytoid 2p68 cells.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 111
- Vial size
- 500 µL
- Concentration
- 0.2 mg/ml
- Storage
- 4° C
Submitted references SMCHD1 accumulates at DNA damage sites and facilitates the repair of DNA double-strand breaks.
Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.
Imatinib radiosensitizes bladder cancer by targeting homologous recombination.
A noncatalytic function of the ligation complex during nonhomologous end joining.
DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1.
Ku80 functions as a tumor suppressor in hepatocellular carcinoma by inducing S-phase arrest through a p53-dependent pathway.
A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia.
The effect of DNA-dependent protein kinase on adeno-associated virus replication.
Involvement of p54(nrb), a PSF partner protein, in DNA double-strand break repair and radioresistance.
Hyperactivation of DNA-PK by double-strand break mimicking molecules disorganizes DNA damage response.
Ku proteins function as corepressors to regulate farnesoid X receptor-mediated gene expression.
Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo.
Cell-surface MMP-9 regulates the invasive capacity of leukemia blast cells with monocytic features.
Cell-surface MMP-9 regulates the invasive capacity of leukemia blast cells with monocytic features.
DNA-dependent conformational changes in the Ku heterodimer.
Transcription-dependent nucleolar cap localization and possible nuclear function of DExH RNA helicase RHAU.
Analytical biochemistry of DNA--protein assemblies from crude cell extracts.
Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell.
Long-term XPC silencing reduces DNA double-strand break repair.
Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection.
Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends.
Impaired DNA double strand break repair in cells from Nijmegen breakage syndrome patients.
Proteomic identification of Ku70/Ku80 autoantigen recognized by monoclonal antibody against hepatocellular carcinoma.
Human chronic lymphocytic leukemia B cells can escape DNA damage-induced apoptosis through the nonhomologous end-joining DNA repair pathway.
Cell cycle-dependent nuclear retention of p53 by E2F1 requires phosphorylation of p53 at Ser315.
The HoxC4 homeodomain protein mediates activation of the immunoglobulin heavy chain 3' hs1,2 enhancer in human B cells. Relevance to class switch DNA recombination.
The membrane form of the DNA repair protein Ku interacts at the cell surface with metalloproteinase 9.
Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining.
Suppression of retroviral infection by the RAD52 DNA repair protein.
DNA damage modulates nucleolar interaction of the Werner protein with the AAA ATPase p97/VCP.
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
Evidence implicating Ku antigen as a structural factor in RNA polymerase II-mediated transcription.
Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment.
Induction of protein translation by ADAR1 within living cell nuclei is not dependent on RNA editing.
Binding of inositol hexakisphosphate (IP6) to Ku but not to DNA-PKcs.
Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
A method to detect particle-specific antibodies against Ku and the DNA-dependent protein kinase catalytic subunit in autoimmune sera.
Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku70 and Ku80 subunits.
Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro.
Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro.
Ku entry into DNA inhibits inward DNA transactions in vitro.
Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.
Poly(ADP-ribose) polymerase and Ku autoantigen form a complex and synergistically bind to matrix attachment sequences.
The repressor which binds the -75 GATA motif of the GPB promoter contains Ku70 as the DNA binding subunit.
Coker H, Brockdorff N
Journal of cell science 2014 May 1;127(Pt 9):1869-74
Journal of cell science 2014 May 1;127(Pt 9):1869-74
Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.
Kruer TL, Cummins TD, Powell DW, Wittliff JL
Clinical biochemistry 2013 Nov;46(16-17):1739-46
Clinical biochemistry 2013 Nov;46(16-17):1739-46
Imatinib radiosensitizes bladder cancer by targeting homologous recombination.
Qiao B, Kerr M, Groselj B, Teo MT, Knowles MA, Bristow RG, Phillips RM, Kiltie AE
Cancer research 2013 Mar 1;73(5):1611-20
Cancer research 2013 Mar 1;73(5):1611-20
A noncatalytic function of the ligation complex during nonhomologous end joining.
Cottarel J, Frit P, Bombarde O, Salles B, Négrel A, Bernard S, Jeggo PA, Lieber MR, Modesti M, Calsou P
The Journal of cell biology 2013 Jan 21;200(2):173-86
The Journal of cell biology 2013 Jan 21;200(2):173-86
DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1.
Calkins AS, Iglehart JD, Lazaro JB
Nucleic acids research 2013 Aug;41(15):7378-86
Nucleic acids research 2013 Aug;41(15):7378-86
Ku80 functions as a tumor suppressor in hepatocellular carcinoma by inducing S-phase arrest through a p53-dependent pathway.
Wei S, Xiong M, Zhan DQ, Liang BY, Wang YY, Gutmann DH, Huang ZY, Chen XP
Carcinogenesis 2012 Mar;33(3):538-47
Carcinogenesis 2012 Mar;33(3):538-47
A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia.
Bouquet F, Ousset M, Biard D, Fallone F, Dauvillier S, Frit P, Salles B, Muller C
Journal of cell science 2011 Jun 1;124(Pt 11):1943-51
Journal of cell science 2011 Jun 1;124(Pt 11):1943-51
The effect of DNA-dependent protein kinase on adeno-associated virus replication.
Choi YK, Nash K, Byrne BJ, Muzyczka N, Song S
PloS one 2010 Dec 20;5(12):e15073
PloS one 2010 Dec 20;5(12):e15073
Involvement of p54(nrb), a PSF partner protein, in DNA double-strand break repair and radioresistance.
Li S, Kuhne WW, Kulharya A, Hudson FZ, Ha K, Cao Z, Dynan WS
Nucleic acids research 2009 Nov;37(20):6746-53
Nucleic acids research 2009 Nov;37(20):6746-53
Hyperactivation of DNA-PK by double-strand break mimicking molecules disorganizes DNA damage response.
Quanz M, Chassoux D, Berthault N, Agrario C, Sun JS, Dutreix M
PloS one 2009 Jul 21;4(7):e6298
PloS one 2009 Jul 21;4(7):e6298
Ku proteins function as corepressors to regulate farnesoid X receptor-mediated gene expression.
Ohno M, Kunimoto M, Nishizuka M, Osada S, Imagawa M
Biochemical and biophysical research communications 2009 Dec 18;390(3):738-42
Biochemical and biophysical research communications 2009 Dec 18;390(3):738-42
Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo.
Yu D, Sekine E, Fujimori A, Ochiya T, Okayasu R
Cancer science 2008 Apr;99(4):810-5
Cancer science 2008 Apr;99(4):810-5
Cell-surface MMP-9 regulates the invasive capacity of leukemia blast cells with monocytic features.
Paupert J, Mansat-De Mas V, Demur C, Salles B, Muller C
Cell cycle (Georgetown, Tex.) 2008 Apr 15;7(8):1047-53
Cell cycle (Georgetown, Tex.) 2008 Apr 15;7(8):1047-53
Cell-surface MMP-9 regulates the invasive capacity of leukemia blast cells with monocytic features.
Paupert J, Mansat-De Mas V, Demur C, Salles B, Muller C
Cell cycle (Georgetown, Tex.) 2008 Apr 15;7(8):1047-53
Cell cycle (Georgetown, Tex.) 2008 Apr 15;7(8):1047-53
DNA-dependent conformational changes in the Ku heterodimer.
Lehman JA, Hoelz DJ, Turchi JJ
Biochemistry 2008 Apr 15;47(15):4359-68
Biochemistry 2008 Apr 15;47(15):4359-68
Transcription-dependent nucleolar cap localization and possible nuclear function of DExH RNA helicase RHAU.
Iwamoto F, Stadler M, Chalupníková K, Oakeley E, Nagamine Y
Experimental cell research 2008 Apr 1;314(6):1378-91
Experimental cell research 2008 Apr 1;314(6):1378-91
Analytical biochemistry of DNA--protein assemblies from crude cell extracts.
Hégarat N, Cardoso GM, Rusconi F, Francois JC, Praseuth D
Nucleic acids research 2007;35(13):e92
Nucleic acids research 2007;35(13):e92
Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell.
Wu PY, Frit P, Malivert L, Revy P, Biard D, Salles B, Calsou P
The Journal of biological chemistry 2007 Nov 2;282(44):31937-43
The Journal of biological chemistry 2007 Nov 2;282(44):31937-43
Long-term XPC silencing reduces DNA double-strand break repair.
Despras E, Pfeiffer P, Salles B, Calsou P, Kuhfittig-Kulle S, Angulo JF, Biard DS
Cancer research 2007 Mar 15;67(6):2526-34
Cancer research 2007 Mar 15;67(6):2526-34
Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection.
Luo MH, Rosenke K, Czornak K, Fortunato EA
Journal of virology 2007 Feb;81(4):1934-50
Journal of virology 2007 Feb;81(4):1934-50
Interplay between Ku, Artemis, and the DNA-dependent protein kinase catalytic subunit at DNA ends.
Drouet J, Frit P, Delteil C, de Villartay JP, Salles B, Calsou P
The Journal of biological chemistry 2006 Sep 22;281(38):27784-93
The Journal of biological chemistry 2006 Sep 22;281(38):27784-93
Impaired DNA double strand break repair in cells from Nijmegen breakage syndrome patients.
Howlett NG, Scuric Z, D'Andrea AD, Schiestl RH
DNA repair 2006 Feb 3;5(2):251-7
DNA repair 2006 Feb 3;5(2):251-7
Proteomic identification of Ku70/Ku80 autoantigen recognized by monoclonal antibody against hepatocellular carcinoma.
Luk JM, Su YC, Lam SC, Lee CK, Hu MY, He QY, Lau GK, Wong FW, Fan ST
Proteomics 2005 May;5(7):1980-6
Proteomics 2005 May;5(7):1980-6
Human chronic lymphocytic leukemia B cells can escape DNA damage-induced apoptosis through the nonhomologous end-joining DNA repair pathway.
Deriano L, Guipaud O, Merle-Béral H, Binet JL, Ricoul M, Potocki-Veronese G, Favaudon V, Maciorowski Z, Muller C, Salles B, Sabatier L, Delic J
Blood 2005 Jun 15;105(12):4776-83
Blood 2005 Jun 15;105(12):4776-83
Cell cycle-dependent nuclear retention of p53 by E2F1 requires phosphorylation of p53 at Ser315.
Fogal V, Hsieh JK, Royer C, Zhong S, Lu X
The EMBO journal 2005 Aug 3;24(15):2768-82
The EMBO journal 2005 Aug 3;24(15):2768-82
The HoxC4 homeodomain protein mediates activation of the immunoglobulin heavy chain 3' hs1,2 enhancer in human B cells. Relevance to class switch DNA recombination.
Kim EC, Edmonston CR, Wu X, Schaffer A, Casali P
The Journal of biological chemistry 2004 Oct 1;279(40):42258-69
The Journal of biological chemistry 2004 Oct 1;279(40):42258-69
The membrane form of the DNA repair protein Ku interacts at the cell surface with metalloproteinase 9.
Monferran S, Paupert J, Dauvillier S, Salles B, Muller C
The EMBO journal 2004 Oct 1;23(19):3758-68
The EMBO journal 2004 Oct 1;23(19):3758-68
Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining.
Audebert M, Salles B, Calsou P
The Journal of biological chemistry 2004 Dec 31;279(53):55117-26
The Journal of biological chemistry 2004 Dec 31;279(53):55117-26
Suppression of retroviral infection by the RAD52 DNA repair protein.
Lau A, Kanaar R, Jackson SP, O'Connor MJ
The EMBO journal 2004 Aug 18;23(16):3421-9
The EMBO journal 2004 Aug 18;23(16):3421-9
DNA damage modulates nucleolar interaction of the Werner protein with the AAA ATPase p97/VCP.
Partridge JJ, Lopreiato JO Jr, Latterich M, Indig FE
Molecular biology of the cell 2003 Oct;14(10):4221-9
Molecular biology of the cell 2003 Oct;14(10):4221-9
Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.
Udayakumar D, Bladen CL, Hudson FZ, Dynan WS
The Journal of biological chemistry 2003 Oct 24;278(43):41631-5
The Journal of biological chemistry 2003 Oct 24;278(43):41631-5
Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.
Onclercq-Delic R, Calsou P, Delteil C, Salles B, Papadopoulo D, Amor-Guéret M
Nucleic acids research 2003 Nov 1;31(21):6272-82
Nucleic acids research 2003 Nov 1;31(21):6272-82
Evidence implicating Ku antigen as a structural factor in RNA polymerase II-mediated transcription.
Bertinato J, Tomlinson JJ, Schild-Poulter C, Haché RJ
Gene 2003 Jan 2;302(1-2):53-64
Gene 2003 Jan 2;302(1-2):53-64
Coordinated assembly of Ku and p460 subunits of the DNA-dependent protein kinase on DNA ends is necessary for XRCC4-ligase IV recruitment.
Calsou P, Delteil C, Frit P, Drouet J, Salles B
Journal of molecular biology 2003 Feb 7;326(1):93-103
Journal of molecular biology 2003 Feb 7;326(1):93-103
Induction of protein translation by ADAR1 within living cell nuclei is not dependent on RNA editing.
Herbert A, Wagner S, Nickerson JA
Molecular cell 2002 Nov;10(5):1235-46
Molecular cell 2002 Nov;10(5):1235-46
Binding of inositol hexakisphosphate (IP6) to Ku but not to DNA-PKcs.
Ma Y, Lieber MR
The Journal of biological chemistry 2002 Mar 29;277(13):10756-9
The Journal of biological chemistry 2002 Mar 29;277(13):10756-9
Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.
Ma Y, Pannicke U, Schwarz K, Lieber MR
Cell 2002 Mar 22;108(6):781-94
Cell 2002 Mar 22;108(6):781-94
A method to detect particle-specific antibodies against Ku and the DNA-dependent protein kinase catalytic subunit in autoimmune sera.
Jafri F, Hardin JA, Dynan WS
Journal of immunological methods 2001 May 1;251(1-2):53-61
Journal of immunological methods 2001 May 1;251(1-2):53-61
Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku70 and Ku80 subunits.
Bertinato J, Schild-Poulter C, Haché RJ
Journal of cell science 2001 Jan;114(Pt 1):89-99
Journal of cell science 2001 Jan;114(Pt 1):89-99
Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro.
Lynch EM, Moreland RB, Ginis I, Perrine SP, Faller DV
American journal of physiology. Cell physiology 2001 Apr;280(4):C897-911
American journal of physiology. Cell physiology 2001 Apr;280(4):C897-911
Hypoxia-activated ligand HAL-1/13 is lupus autoantigen Ku80 and mediates lymphoid cell adhesion in vitro.
Lynch EM, Moreland RB, Ginis I, Perrine SP, Faller DV
American journal of physiology. Cell physiology 2001 Apr;280(4):C897-911
American journal of physiology. Cell physiology 2001 Apr;280(4):C897-911
Ku entry into DNA inhibits inward DNA transactions in vitro.
Frit P, Li RY, Arzel D, Salles B, Calsou P
The Journal of biological chemistry 2000 Nov 17;275(46):35684-91
The Journal of biological chemistry 2000 Nov 17;275(46):35684-91
Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.
Chen L, Trujillo K, Sung P, Tomkinson AE
The Journal of biological chemistry 2000 Aug 25;275(34):26196-205
The Journal of biological chemistry 2000 Aug 25;275(34):26196-205
Mutations in human ARF exon 2 disrupt its nucleolar localization and impair its ability to block nuclear export of MDM2 and p53.
Zhang Y, Xiong Y
Molecular cell 1999 May;3(5):579-91
Molecular cell 1999 May;3(5):579-91
Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.
Giffin W, Gong W, Schild-Poulter C, Haché RJ
Molecular and cellular biology 1999 Jun;19(6):4065-78
Molecular and cellular biology 1999 Jun;19(6):4065-78
Poly(ADP-ribose) polymerase and Ku autoantigen form a complex and synergistically bind to matrix attachment sequences.
Galande S, Kohwi-Shigematsu T
The Journal of biological chemistry 1999 Jul 16;274(29):20521-8
The Journal of biological chemistry 1999 Jul 16;274(29):20521-8
The repressor which binds the -75 GATA motif of the GPB promoter contains Ku70 as the DNA binding subunit.
Camara-Clayette V, Thomas D, Rahuel C, Barbey R, Cartron JP, Bertrand O
Nucleic acids research 1999 Apr 1;27(7):1656-63
Nucleic acids research 1999 Apr 1;27(7):1656-63
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Ku (P80) using Ku (P80) Monoclonal Antibody (Product # MA5-12930) on Native Human T47D Cells.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Ku (P80) using Ku (P80) Monoclonal Antibody (Product # MA5-12930) on denatured Human BT474 Cells.
- Conjugate
- Biotin
