GTX70274

antibody from GeneTex

Targeting: XRCC5 KARP-1, KU80, Ku86, KUB2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX70274

Provider

GeneTex

Proper citation

GeneTex Cat#GTX70274, RRID:AB_372051

Product name

Ku80 antibody [9403]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

GTX70274 at a 1/2000 dilution staining ~ 80 kDa Ku80 in 35mg of T24 whole cell extract. ECL detection.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug D: 293T 1ug 7.5% SDS PAGE GTX70274 diluted at 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE GTX70274 diluted at 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed HeLa, using Ku80(GTX70274) antibody (green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2000.