GTX70276

antibody from GeneTex

Targeting: XRCC5 KARP-1, KU80, Ku86, KUB2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX70276

Provider

GeneTex

Proper citation

GeneTex Cat#GTX70276, RRID:AB_372053

Product name

Ku80 antibody [149.8]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

Localization of Double-Strand Break Repair Proteins to Viral Replication Compartments following Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus.

Hollingworth R, Horniblow RD, Forrest C, Stewart GS, Grand RJ
Journal of virology 2017 Nov 15;91(22)

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DNA-PK/Chk2 induces centrosome amplification during prolonged replication stress.

Wang CY, Huang EY, Huang SC, Chung BC
Oncogene 2015 Mar 5;34(10):1263-9

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Detection of human Ku80 protein in HeLa whole cell lysate using GeneTex Ku80 149.8 monoclonal antibody GTX70276.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE GTX70276 diluted at 1:10000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7.5% SDS PAGE GTX70276 diluted at 1:10000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Ku80 antibody [149.8] detects Ku80 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ku80 protein stained by Ku80 antibody [149.8] (GTX70276) diluted at 1:500.Blue: Hoechst 33343 staining.