PA5-89161

antibody from Invitrogen Antibodies

Targeting: LRP6 ADCAD2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89161

Provider

Invitrogen Antibodies

Product name

LRP6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: APLLLYANRR DLRLVDATNG KENATIVVGG LEDAAAVDFV FSHGLIYWSD VSEEAIKRTE FNKTESVQNV VVSGLLSPDG LACDWLGEKL YWTDSETNRI EVSNLDGSLR KVLFWQELDQ PRAIALDPSS G; Positive Samples: Mouse liver; Cellular Location: Endoplasmic reticulum, Membrane, Single-pass type I membrane protein

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.14 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

HLY78 Attenuates Neuronal Apoptosis via the LRP6/GSK3β/β-Catenin Signaling Pathway After Subarachnoid Hemorrhage in Rats.

Luo X, Li L, Xu W, Cheng Y, Xie Z
Neuroscience bulletin 2020 Oct;36(10):1171-1181

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of LRP6 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with LRP6 Polyclonal Antibody (Product # PA5-89161) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Alexa Fluor™ Plus 647 Phalloidin (Product # A30107, 1:2000 dilution). Panel d represents the merged image showing endoplasmic reticulum localization. Panel e represents control cells with no primary antibody to assess the background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7A1110LZR).