Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF6649 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-LRP-6 (S1490) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LRP-6 when phosphorylated at S1490 in Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Activation of intracellular calcium by multiple Wnt ligands and translocation of β-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/β-catenin pathways.
Thrasivoulou C, Millar M, Ahmed A
The Journal of biological chemistry 2013 Dec 13;288(50):35651-9
The Journal of biological chemistry 2013 Dec 13;288(50):35651-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Phospho-LRP-6 (S1490) by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line transfected with human LRP-6 untreated (-) or treated (+) with 100 ng/mL Recombinant Human Wnt-3a (High Purity) (Catalog # 5036-WNP) for 15 minutes. PVDF Membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-LRP-6 (S1490) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6649) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-LRP-6 (S1490) at approximately 200 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 300 U/mL CIP for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.