18222-1-AP

antibody from Proteintech Group

Targeting: NFATC3 NFAT4, NFATX

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18222-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18222-1-AP, RRID:AB_2152773

Product name

NFATC3 antibody

Antibody type

Polyclonal

Description

KD/KO validated NFATC3 antibody (Cat. #18222-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Dual action of macrophage miR-204 confines cyclosporine A-induced atherosclerosis.

Su JH, Hong Y, Han CC, Yu J, Guan X, Zhu YM, Wang C, Ma MM, Pang RP, Ou JS, Zhou JG, Zhang ZY, Ban T, Liang SJ
British journal of pharmacology 2024 Mar;181(5):640-658

---

Dephosphorylation of NFAT by Calcineurin inhibits Skp2-mediated degradation.

Hanaki S, Habara M, Sato Y, Tomiyasu H, Miki Y, Shibutani S, Shimada M
Journal of biochemistry 2024 Mar 4;175(3):235-244

---

miR-339-3p promotes AT1-AA-induced vascular inflammation by upregulating NFATc3 protein expression in vascular smooth muscle cells.

Li Y, Long Y, Zhi X, Hao H, Wang X, Liu H, Wang L
Acta biochimica et biophysica Sinica 2023 Feb 25;55(2):295-303

---

Blocking Store-Operated Ca(2+) Entry to Protect HL-1 Cardiomyocytes from Epirubicin-Induced Cardiotoxicity.

Liu X, Chang Y, Choi S, Cai C, Zhang X, Pan Z
Cells 2023 Feb 24;12(5)

---

Substitution of SERCA2 Cys(674) aggravates cardiac fibrosis by promoting the transformation of cardiac fibroblasts to cardiac myofibroblasts.

Huang X, Lin X, Wang L, Xie Y, Que Y, Li S, Hu P, Tong X
Biochemical pharmacology 2022 Sep;203:115164

---

Pou3f1 mediates the effect of Nfatc3 on ulcerative colitis-associated colorectal cancer by regulating inflammation.

Lin Y, Wang D, Zhao H, Li D, Li X, Lin L
Cellular & molecular biology letters 2022 Sep 5;27(1):75

---

Trim39 regulates neuronal apoptosis by acting as a SUMO-targeted E3 ubiquitin-ligase for the transcription factor NFATc3.

Basu-Shrivastava M, Mojsa B, Mora S, Robbins I, Bossis G, Lassot I, Desagher S
Cell death and differentiation 2022 Nov;29(11):2107-2122

---

A-kinase anchoring protein 5-ancored calcineurin regulates the remodeling of H9c2 cardiomyocytes exposed to hypoxia and reoxygenation.

Zhu F, Yuan C, Zhang X, Wang Z, Wang Q, Wang H
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022 Nov;155:113689

---

TARBP2-stablized SNHG7 regulates blood-brain barrier permeability by acting as a competing endogenous RNA to miR-17-5p/NFATC3 in Aβ-microenvironment.

Ning H, Zhang L, Zhu B, Zhou X, Zhang T, Ma T
Cell death & disease 2022 May 13;13(5):457

---

Trans-cinnamaldehyde suppresses microtubule detyrosination and alleviates cardiac hypertrophy.

Tian J, Shan XL, Wang SN, Chen HH, Zhao P, Qian DD, Xu M, Guo W, Zhang C, Lu R
European journal of pharmacology 2022 Jan 5;914:174687

---

Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.

Li X, Zhang Y, Zhao Y, Zhou Y, Han Q, Yang Y, Zhang L, Shi L, Jin X, Zhang R, Gao H, Xue G, Li D, Zhang ZR, Lu Y, Yang B, Pan Z
Pharmacological research 2022 Aug;182:106284

---

Transmembrane Protein Ttyh1 Maintains the Quiescence of Neural Stem Cells Through Ca(2+)/NFATc3 Signaling.

Cao Y, Wu HN, Cao XL, Yue KY, Han WJ, Cao ZP, Zhang YF, Gao XY, Luo C, Jiang XF, Han H, Zheng MH
Frontiers in cell and developmental biology 2021;9:779373

---

TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.

Lin DC, Zheng SY, Zhang ZG, Luo JH, Zhu ZL, Li L, Chen LS, Lin X, Sham JSK, Lin MJ, Zhou RX
Cancer letters 2021 Oct 28;519:211-225

---

The role of nuclear receptor 4A1 (NR4A1) in drug-induced gingival overgrowth.

Hatano S, Matsuda S, Okanobu A, Furutama D, Memida T, Kajiya M, Ouhara K, Fujita T, Mizuno N, Kurihara H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Jul;35(7):e21693

---

Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Xian H, Liu Y, Rundberg Nilsson A, Gatchalian R, Crother TR, Tourtellotte WG, Zhang Y, Aleman-Muench GR, Lewis G, Chen W, Kang S, Luevanos M, Trudler D, Lipton SA, Soroosh P, Teijaro J, de la Torre JC, Arditi M, Karin M, Sanchez-Lopez E
Immunity 2021 Jul 13;54(7):1463-1477.e11

---

Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.

Lao M, Zhang X, Ma T, Xu J, Yang H, Duan Y, Ying H, Zhang X, Guo C, Qiu J, Bai X, Liang T
Oncogene 2021 Apr;40(17):3136-3151

---

Protective Role of Tangshen Formula on the Progression of Renal Damage in db/db Mice by TRPC6/Talin1 Pathway in Podocytes.

Wang Q, Tian X, Zhou W, Wang Y, Zhao H, Li J, Zhou X, Zhang H, Zhao T, Li P
Journal of diabetes research 2020;2020:3634974

---

Tsantan Sumtang Restored Right Ventricular Function in Chronic Hypoxia-Induced Pulmonary Hypertension Rats.

Yang Z, Sun H, Su S, Nan X, Li K, Jin X, Jin G, Li Z, Lu D
Frontiers in pharmacology 2020;11:607384

---

Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.

Zheng J, Tian J, Wang S, Hu P, Wu Q, Shan X, Zhao P, Zhang C, Guo W, Xu M, Chen H, Lu R
European journal of pharmacology 2020 Sep 15;883:173386

---

Blocking NFATc3 ameliorates azoxymethane/dextran sulfate sodium induced colitis-associated colorectal cancer in mice via the inhibition of inflammatory responses and epithelial-mesenchymal transition.

Lin Y, Koumba MH, Qu S, Wang D, Lin L
Cellular signalling 2020 Oct;74:109707

---

Methionine Enkephalin Suppresses Osteocyte Apoptosis Induced by Compressive Force through Regulation of Nuclear Translocation of NFATc1.

Sogi C, Takeshita N, Jiang W, Kim S, Maeda T, Yoshida M, Oyanagi T, Ito A, Kimura S, Seki D, Takano I, Sakai Y, Fujiwara I, Kure S, Takano-Yamamoto T
JBMR plus 2020 Jul;4(7):e10369

---

The calcium pump PMCA4 prevents epithelial-mesenchymal transition by inhibiting NFATc1-ZEB1 pathway in gastric cancer.

Wang T, Li N, Jin L, Qi X, Zhang C, Hua D
Biochimica et biophysica acta. Molecular cell research 2020 Dec;1867(12):118833

---

NFATc3 regulation of collagen V expression contributes to cellular immunity to collagen type V and hypoxic pulmonary hypertension.

Sheak JR, Jones DT, Lantz BJ, Maston LD, Vigil D, Resta TC, Resta MM, Howard TA, Kanagy NL, Guo Y, Jankowska-Gan E, Sullivan JA, Braun RK, Burlingham WJ, Gonzalez Bosc LV
American journal of physiology. Lung cellular and molecular physiology 2020 Dec 1;319(6):L968-L980

---

Calcineurin/NFAT Signaling Modulates Pulmonary Artery Smooth Muscle Cell Proliferation, Migration and Apoptosis in Monocrotaline-Induced Pulmonary Arterial Hypertension Rats.

He RL, Wu ZJ, Liu XR, Gui LX, Wang RX, Lin MJ
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;49(1):172-189

---

TRPV2-induced Ca(2+)-calcineurin-NFAT signaling regulates differentiation of osteoclast in multiple myeloma.

Bai H, Zhu H, Yan Q, Shen X, Lu X, Wang J, Li J, Chen L
Cell communication and signaling : CCS 2018 Oct 16;16(1):68

---

Regulator of Calcineurin 1 Gene Isoform 4, Down-regulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Metastasis of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.

Jin H, Wang C, Jin G, Ruan H, Gu D, Wei L, Wang H, Wang N, Arunachalam E, Zhang Y, Deng X, Yang C, Xiong Y, Feng H, Yao M, Fang J, Gu J, Cong W, Qin W
Gastroenterology 2017 Sep;153(3):799-811.e33

---

Nuclear factor of activated T-cell c3 inhibition of mammalian target of rapamycin signaling through induction of regulated in development and DNA damage response 1 in human intestinal cells.

Zhou Y, Wang Q, Guo Z, Weiss HL, Evers BM
Molecular biology of the cell 2012 Aug;23(15):2963-72

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18222-1-AP( NFATC3 Antibody) at dilution of 1:300

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The NFATC3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NFATC3. This antibody recognizes human, mouse antigen. The NFATC3 antibody has been validated for the following applications: ELISA, IHC, WB analysis.

Appendix

18222-1-AP.pdf

Show more

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 18222-1-AP(NFATC3 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue