PA5-36101

antibody from Invitrogen Antibodies

Targeting: NFATC3 NFAT4, NFATX

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36101

Provider

Invitrogen Antibodies

Product name

NFATC3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 115 kDa. Purity is >95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Myocardial Hypertrophy and Fibrosis Are Associated with Cardiomyocyte Beta-Catenin and TRPC6/Calcineurin/NFAT Signaling in Spontaneously Hypertensive Rats with 5/6 Nephrectomy.

Bogdanova E, Beresneva O, Galkina O, Zubina I, Ivanova G, Parastaeva M, Semenova N, Dobronravov V
International journal of molecular sciences 2021 Apr 28;22(9)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFAT4 using NFAT4 polyclonal antibody (Product # PA5-36101) at a dilution of 1:500. Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NFATC3 in Lane 1: PC3 whole cell lysate (60?g), Lane 2: the Testis tissue lysate of mouse (60?g), Lane 3: the Testis tissue lysate of rat (60?g). Samples were incubated with NFATC3 polyclonal antibody (Product # PA5-36101) at a dilution of 1:500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Myocardial expression of calcineurin/NFAT and TRPC6: ( a ) the representative microphotographs of the myocardial expression of TRPC6/CaN/NFAT; the morphometric analysis of myocardial ( b ) TRPC6 (200 x ; scale bar, 100 mum), ( c ) CaN (200 x ), and ( d ) nuclear NFATc3 (400 x ; scale bar, 50 mum) IHC staining; and ( c ) the relative mRNA expression of ( e ) Trpc6 , ( f ) calcineurin A ( Ppp3ca ) and ( g ) calcineurin B ( Ppp3r1 ) in controls, hypertension, and renal dysfunction; FOV--field of view; Nu--nuclei.