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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP2-04027 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal NFATC3/NFAT4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NFATC3/NFAT4 Antibody [NBP2-04027] - Detection of human NFATc3 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04027 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04025 and NBP2-04026, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NFAT4 Antibody [NBP2-04027] - Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04025 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04026 and NBP2-04027, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
