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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- 600-401-A20 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-A20, RRID:AB_2299606
- Product name
- Anti-PIN1 [RABBIT] Antibody - 600-401-A20
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor α in breast cancer.
Dissecting Pin1 and phospho-pRb regulation.
Androgen receptor serine 81 mediates Pin1 interaction and activity.
Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1.
Lucchetti C, Caligiuri I, Toffoli G, Giordano A, Rizzolio F
PloS one 2013;8(2):e55355
PloS one 2013;8(2):e55355
Dissecting Pin1 and phospho-pRb regulation.
Rizzolio F, Caligiuri I, Lucchetti C, Fratamico R, Tomei V, Gallo G, Agelan A, Ferrari G, Toffoli G, Klein-Szanto AJ, Giordano A
Journal of cellular physiology 2013 Jan;228(1):73-7
Journal of cellular physiology 2013 Jan;228(1):73-7
Androgen receptor serine 81 mediates Pin1 interaction and activity.
La Montagna R, Caligiuri I, Maranta P, Lucchetti C, Esposito L, Paggi MG, Toffoli G, Rizzolio F, Giordano A
Cell cycle (Georgetown, Tex.) 2012 Sep 15;11(18):3415-20
Cell cycle (Georgetown, Tex.) 2012 Sep 15;11(18):3415-20
Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1.
Rizzolio F, Lucchetti C, Caligiuri I, Marchesi I, Caputo M, Klein-Szanto AJ, Bagella L, Castronovo M, Giordano A
Cell death and differentiation 2012 Jul;19(7):1152-61
Cell death and differentiation 2012 Jul;19(7):1152-61
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-Pin1 antibody to detect endogenous Pin1 in HeLa whole cell lysates. The sample was run in duplicate. A band representing Pin1 is indicated by the arrowhead. Cell lysates were electrophoresed using a straight 15% polyacrylamide gel, followed by transfer to nitrocellulose. The membrane was probed with the primary antibody at a 1:700 dilution. A 1:5,000 dilution of HRP Gt-a-Rabbit IgG (611-103-122) was used with a 15 sec exposure time. Personal Communication, L. D'agostino and A. Giordano, SHRO, Philadelphia, PA.
- Validation comment
- Western Blot
