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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-7482 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-7482, RRID:AB_628177
- Product name
- Anti-PPP1CA
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Tauopathic changes in the striatum of A53T α-synuclein mutant mouse model of Parkinson's disease.
An overlapping kinase and phosphatase docking site regulates activity of the retinoblastoma protein.
Protein phosphatase 1γ is responsible for dephosphorylation of histone H3 at Thr 11 after DNA damage.
Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis.
More severe cellular phenotype in human idiopathic dilated cardiomyopathy compared to ischemic heart disease.
Ikaros stability and pericentromeric localization are regulated by protein phosphatase 1.
A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin.
Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
Protein phosphatase 1 regulates the stability of the circadian protein PER2.
Central role for protein targeting to glycogen in the maintenance of cellular glycogen stores in 3T3-L1 adipocytes.
Control of alpha subunit of eukaryotic translation initiation factor 2 (eIF2 alpha) phosphorylation by the human papillomavirus type 18 E6 oncoprotein: implications for eIF2 alpha-dependent gene expression and cell death.
Wills J, Credle J, Haggerty T, Lee JH, Oaks AW, Sidhu A
PloS one 2011 Mar 21;6(3):e17953
PloS one 2011 Mar 21;6(3):e17953
An overlapping kinase and phosphatase docking site regulates activity of the retinoblastoma protein.
Hirschi A, Cecchini M, Steinhardt RC, Schamber MR, Dick FA, Rubin SM
Nature structural & molecular biology 2010 Sep;17(9):1051-7
Nature structural & molecular biology 2010 Sep;17(9):1051-7
Protein phosphatase 1γ is responsible for dephosphorylation of histone H3 at Thr 11 after DNA damage.
Shimada M, Haruta M, Niida H, Sawamoto K, Nakanishi M
EMBO reports 2010 Nov;11(11):883-9
EMBO reports 2010 Nov;11(11):883-9
Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis.
Wang Z, Udeshi ND, Slawson C, Compton PD, Sakabe K, Cheung WD, Shabanowitz J, Hunt DF, Hart GW
Science signaling 2010 Jan 12;3(104):ra2
Science signaling 2010 Jan 12;3(104):ra2
More severe cellular phenotype in human idiopathic dilated cardiomyopathy compared to ischemic heart disease.
Hamdani N, Borbély A, Veenstra SP, Kooij V, Vrydag W, Zaremba R, Dos Remedios C, Niessen HW, Michel MC, Paulus WJ, Stienen GJ, van der Velden J
Journal of muscle research and cell motility 2010 Dec;31(4):289-301
Journal of muscle research and cell motility 2010 Dec;31(4):289-301
Ikaros stability and pericentromeric localization are regulated by protein phosphatase 1.
Popescu M, Gurel Z, Ronni T, Song C, Hung KY, Payne KJ, Dovat S
The Journal of biological chemistry 2009 May 15;284(20):13869-13880
The Journal of biological chemistry 2009 May 15;284(20):13869-13880
A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin.
Slawson C, Lakshmanan T, Knapp S, Hart GW
Molecular biology of the cell 2008 Oct;19(10):4130-40
Molecular biology of the cell 2008 Oct;19(10):4130-40
Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
Caldas H, Fangusaro JR, Boué DR, Holloway MP, Altura RA
Blood 2007 Feb 15;109(4):1479-89
Blood 2007 Feb 15;109(4):1479-89
Protein phosphatase 1 regulates the stability of the circadian protein PER2.
Gallego M, Kang H, Virshup DM
The Biochemical journal 2006 Oct 1;399(1):169-75
The Biochemical journal 2006 Oct 1;399(1):169-75
Central role for protein targeting to glycogen in the maintenance of cellular glycogen stores in 3T3-L1 adipocytes.
Greenberg CC, Danos AM, Brady MJ
Molecular and cellular biology 2006 Jan;26(1):334-42
Molecular and cellular biology 2006 Jan;26(1):334-42
Control of alpha subunit of eukaryotic translation initiation factor 2 (eIF2 alpha) phosphorylation by the human papillomavirus type 18 E6 oncoprotein: implications for eIF2 alpha-dependent gene expression and cell death.
Kazemi S, Papadopoulou S, Li S, Su Q, Wang S, Yoshimura A, Matlashewski G, Dever TE, Koromilas AE
Molecular and cellular biology 2004 Apr;24(8):3415-29
Molecular and cellular biology 2004 Apr;24(8):3415-29
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 39
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
