MAB6105

antibody from Novus Biologicals

Targeting: PPP1CA PP-1A, PP1A, PP1alpha, PPP1A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6105

Provider

Novus Biologicals

Product name

Mouse Monoclonal PP1 alpha/PPP1A Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human PP1 alpha Catalytic Subunit in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) PP1 beta or rhPP1 gamma is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Lymphocyte-specific protein 1 regulates mechanosensory oscillation of podosomes and actin isoform-based actomyosin symmetry breaking.

Cervero P, Wiesner C, Bouissou A, Poincloux R, Linder S
Nature communications 2018 Feb 6;9(1):515

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human PP1 alpha by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, CHP-100 human neuroblastoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PP1 alpha Catalytic Subunit Monoclonal Antibody (Catalog # MAB6105) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human PP1 alpha , recombinant human PP1 beta , and recombinant human PP1 gamma (5 ng/lane) were included. A specific band was detected for PP1 alpha at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.