Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- Ab122816 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- PPP1A/PPP1CA Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human PPP1A/PPP1CA Antibody, Monoclonal (1H1-F7-D8), could be used for WB, ICC/IF and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200Protein FunctionProtein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-330
- Antibody clone number
- 1H1-F7-D8
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PPP1A/PPP1CA Mouse mAb (Ab122816) - Western Blot All lanes: PPP1A/PPP1CA Mouse mAb (Ab122816) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 38 kDa Observed band size: 35, 40 kDa Exposure time: 2.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PPP1A/PPP1CA Mouse mAb (Ab122816) - IF/ICC Immunocytochemistry analysis of PP1C alpha in HeLa cells using PPP1A/PPP1CA Mouse mAb (Ab122816).