MA5-15589

antibody from Invitrogen Antibodies

Targeting: PPP1CA PP-1A, PP1A, PP1alpha, PPP1A

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15589

Provider

Invitrogen Antibodies

Product name

PP1 alpha Monoclonal Antibody (6D1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15589 targets PPP1A in WB applications and shows reactivity with Human and mouse samples.

Antibody clone number

6D1

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-PP1 alpha Monoclonal Antibody (6D1)(Product # MA5-15589) and a 38kDa band corresponding to PP1 alpha was observed across all the tested cell lines and tissues. Whole Cell Extract-WCL (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), A549 (Lane 3), PC-3 (Lane 4), Hep G2 (Lane 5), T98G (Lane 6), HEL 92.1.7 (Lane 7), Raji (Lane 8), THP-1 (Lane 9), Mouse Spleen (Lane 10) and Rat Spleen (Lane 11) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1/1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1/4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PPP1A using PPP1A monoclonal antibody (Product # MA5-15589) in HeLa (1) and NIH/3T3 (2) cell lysate.