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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF7555 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TGIF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TGIF1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q15583- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Secretome and degradome profiling shows that Kallikrein-related peptidases 4, 5, 6, and 7 induce TGFβ-1 signaling in ovarian cancer cells.
Shahinian H, Loessner D, Biniossek ML, Kizhakkedathu JN, Clements JA, Magdolen V, Schilling O
Molecular oncology 2014 Feb;8(1):68-82
Molecular oncology 2014 Feb;8(1):68-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human TGIF1 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human TGIF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7555) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for TGIF1 at approximately 32-34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
