Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 20549-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#20549-1-AP, RRID:AB_10700004
- Product name
- TOP2B antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated TOP2B antibody (Cat. #20549-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references MDM2 provides TOP2 poison resistance by promoting proteolysis of TOP2βcc in a p53-independent manner.
XPF interacts with TOP2B for R-loop processing and DNA looping on actively transcribed genes.
Short-Term Starvation Weakens the Efficacy of Cell Cycle Specific Chemotherapy Drugs through G1 Arrest.
DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells.
Aconitine linoleate, a natural lipo-diterpenoid alkaloid, stimulates anti-proliferative activity reversing doxorubicin resistance in MCF-7/ADR breast cancer cells as a selective topoisomerase IIα inhibitor.
MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".
Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.
Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing.
Etoposide and topoisomerase II inhibition for aggressive prostate cancer: Data from a translational study.
Increasing the distance between two monomers of topoisomerase IIβ under the action of antitumor agent 4β-sulfur-(benzimidazole) 4'-demethylepipodophyllotoxin.
Nuclear localization of Beclin 1 promotes radiation-induced DNA damage repair independent of autophagy.
Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells.
Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.
Shu J, Jiang J, Wang X, Yang X, Zhao G, Cai T
Cell death & disease 2024 Jan 23;15(1):83
Cell death & disease 2024 Jan 23;15(1):83
XPF interacts with TOP2B for R-loop processing and DNA looping on actively transcribed genes.
Chatzinikolaou G, Stratigi K, Siametis A, Goulielmaki E, Akalestou-Clocher A, Tsamardinos I, Topalis P, Austin C, Bouwman BAM, Crosetto N, Altmüller J, Garinis GA
Science advances 2023 Nov 10;9(45):eadi2095
Science advances 2023 Nov 10;9(45):eadi2095
Short-Term Starvation Weakens the Efficacy of Cell Cycle Specific Chemotherapy Drugs through G1 Arrest.
Shi M, Hou J, Shao S, Liang W, Wang S, Yang Y, Guo Z, Pan F
International journal of molecular sciences 2023 Jan 28;24(3)
International journal of molecular sciences 2023 Jan 28;24(3)
DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells.
David M, Olender T, Mizrahi O, Weingarten-Gabbay S, Friedlander G, Meril S, Goldberg N, Savidor A, Levin Y, Salomon V, Stern-Ginossar N, Bialik S, Kimchi A
RNA (New York, N.Y.) 2022 Oct;28(10):1325-1336
RNA (New York, N.Y.) 2022 Oct;28(10):1325-1336
Aconitine linoleate, a natural lipo-diterpenoid alkaloid, stimulates anti-proliferative activity reversing doxorubicin resistance in MCF-7/ADR breast cancer cells as a selective topoisomerase IIα inhibitor.
Luan S, Gao Y, Liang X, Zhang L, Wu Q, Hu Y, Yin L, He C, Liu S
Naunyn-Schmiedeberg's archives of pharmacology 2022 Jan;395(1):65-76
Naunyn-Schmiedeberg's archives of pharmacology 2022 Jan;395(1):65-76
MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".
Das SK, Kuzin V, Cameron DP, Sanford S, Jha RK, Nie Z, Rosello MT, Holewinski R, Andresson T, Wisniewski J, Natsume T, Price DH, Lewis BA, Kouzine F, Levens D, Baranello L
Molecular cell 2022 Jan 6;82(1):140-158.e12
Molecular cell 2022 Jan 6;82(1):140-158.e12
Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.
Zhou Z, Li Y, Ma X, Cao B, Peng T, Sheng Y, Peng H, Li R, Cao Y, Xi R, Li F, Wang M, Sun H, Zhang G, Zhang H, Hu K, Xiao W, Wang F
Journal of medicinal chemistry 2021 Jun 10;64(11):7404-7421
Journal of medicinal chemistry 2021 Jun 10;64(11):7404-7421
Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing.
Herrero-Ruiz A, Martínez-García PM, Terrón-Bautista J, Millán-Zambrano G, Lieberman JA, Jimeno-González S, Cortés-Ledesma F
Cell reports 2021 Apr 13;35(2):108977
Cell reports 2021 Apr 13;35(2):108977
Etoposide and topoisomerase II inhibition for aggressive prostate cancer: Data from a translational study.
Cattrini C, Capaia M, Boccardo F, Barboro P
Cancer treatment and research communications 2020;25:100221
Cancer treatment and research communications 2020;25:100221
Increasing the distance between two monomers of topoisomerase IIβ under the action of antitumor agent 4β-sulfur-(benzimidazole) 4'-demethylepipodophyllotoxin.
Sun LY, Zhu LW, Tang YJ
Scientific reports 2018 Oct 8;8(1):14949
Scientific reports 2018 Oct 8;8(1):14949
Nuclear localization of Beclin 1 promotes radiation-induced DNA damage repair independent of autophagy.
Xu F, Fang Y, Yan L, Xu L, Zhang S, Cao Y, Xu L, Zhang X, Xie J, Jiang G, Ge C, An N, Zhou D, Yuan N, Wang J
Scientific reports 2017 Mar 27;7:45385
Scientific reports 2017 Mar 27;7:45385
Chlorpyrifos Induces MLL Translocations Through Caspase 3-Dependent Genomic Instability and Topoisomerase II Inhibition in Human Fetal Liver Hematopoietic Stem Cells.
Lu C, Liu X, Liu C, Wang J, Li C, Liu Q, Li Y, Li S, Sun S, Yan J, Shao J
Toxicological sciences : an official journal of the Society of Toxicology 2015 Oct;147(2):588-606
Toxicological sciences : an official journal of the Society of Toxicology 2015 Oct;147(2):588-606
Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.
Minajigi A, Froberg J, Wei C, Sunwoo H, Kesner B, Colognori D, Lessing D, Payer B, Boukhali M, Haas W, Lee JT
Science (New York, N.Y.) 2015 Jul 17;349(6245)
Science (New York, N.Y.) 2015 Jul 17;349(6245)
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP(TOP2B antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using TOP2B antibody 20549-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human cervical cancer using 20549-1-AP(TOP2B antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human cervical cancer using 20549-1-AP(TOP2B antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
