Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-89527 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89527, RRID:AB_11035093
- Product name
- Rabbit Polyclonal TOP2B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human TOP2B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TOP2β-Dependent Nuclear DNA Damage Shapes Extracellular Growth Factor Responses via Dynamic AKT Phosphorylation to Control Virus Latency.
HSF1 mediated TNF-α production during proteotoxic stress response pioneers proinflammatory signal in human cells.
Uncovering Hidden Layers of Cell Cycle Regulation through Integrative Multi-omic Analysis.
Hu HL, Shiflett LA, Kobayashi M, Chao MV, Wilson AC, Mohr I, Huang TT
Molecular cell 2019 May 2;74(3):466-480.e4
Molecular cell 2019 May 2;74(3):466-480.e4
HSF1 mediated TNF-α production during proteotoxic stress response pioneers proinflammatory signal in human cells.
Ali A, Biswas A, Pal M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Feb;33(2):2621-2635
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Feb;33(2):2621-2635
Uncovering Hidden Layers of Cell Cycle Regulation through Integrative Multi-omic Analysis.
Aviner R, Shenoy A, Elroy-Stein O, Geiger T
PLoS genetics 2015 Oct;11(10):e1005554
PLoS genetics 2015 Oct;11(10):e1005554
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: TOP2B Antibody [NBP1-89527] - Simple Western lane view shows a specific band for TOP2B in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: TOP2B Antibody [NBP1-89527] - Electropherogram image(s) of corresponding Simple Western lane view. TOP2B antibody was used at 1:100 dilution on RT-4 lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOP2B Antibody [NBP1-89527] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOP2B Antibody [NBP1-89527] - Analysis using Anti-TOP2B antibody NBP1-89527 (A) shows similar pattern to independent antibody NBP2-58728 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human pancreas shows moderate nuclear positivity.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human pancreas shows weak to moderate nuclear positivity in islets of Langerhans and exocrine glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human gastrointestinal shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOP2B Antibody [NBP1-89527] - Staining of human kidney shows moderate nuclear positivity in cells in glomeruli and tubules.