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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-27750 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TOP2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, K562, THP-1.
- Concentration
- 0.48 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TOP2B was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a TOP2B Polyclonal Antibody (Product # PA5-27750) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TOP2B was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a TOP2B Polyclonal Antibody (Product # PA5-27750) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TOP2B Polyclonal Antibody (Product # PA5-27750). Sample (30 µg of whole cell lysate). Lane A: Jurkat. 5% SDS PAGE. TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TOP2B was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using Topoisomerase II beta (Product # PA5-27750) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TOP2B Polyclonal Antibody detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Topoisomerase II beta stained by TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TOP2B Polyclonal Antibody detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Topoisomerase II beta stained by TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TOP2B Polyclonal Antibody detects Topoisomerase II beta protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Topoisomerase II beta stained by TOP2B Polyclonal Antibody (Product # PA5-27750) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Topoisomerase II beta antibody immunoprecipitates Topoisomerase II beta protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of Topoisomerase II beta protein by 2 µg of Topoisomerase II beta antibody (Product # PA5-27750) 5% SDS-PAGE The immunoprecipitated Topoisomerase II beta protein was detected by Topoisomerase II beta antibody (Product # PA5-27750) diluted at 1:1,000.
