M00185-1

antibody from Boster Biological Technology

Targeting: TRAF6 RNF85

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00185-1

Provider

Boster Biological Technology

Product name

Anti-TRAF6 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for TRAF-6/TRAF6 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IHC, WB. Reactive species: Human, Mouse, Rat TRAF-6/TRAF6 information: Molecular Weight: 59573 MW; Subcellular Localization: Cytoplasm . Cytoplasm, cell cortex . Nucleus . Lipid droplet . Found in the nuclei of some aggressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T- and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. RSAD2/viperin recruits it to the lipid droplet (By similarity); Tissue Specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

BOF-20

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TRAF6 Antibody(M00185-1)TRAF6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TRAF6 Antibody (M00185-1)overnight at 4°C Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image