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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AF5479 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TrkA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat TrkA when phosphorylated at Y785 in Western blots. Reactivity with human Phospho-TrkA is expected due to 100% homology with the immunogen.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Nerve growth factor downregulates inflammatory response in human monocytes through TrkA.
Deoxygedunin, a natural product with potent neurotrophic activity in mice.
Prencipe G, Minnone G, Strippoli R, De Pasquale L, Petrini S, Caiello I, Manni L, De Benedetti F, Bracci-Laudiero L
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3345-54
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3345-54
Deoxygedunin, a natural product with potent neurotrophic activity in mice.
Jang SW, Liu X, Chan CB, France SA, Sayeed I, Tang W, Lin X, Xiao G, Andero R, Chang Q, Ressler KJ, Ye K
PloS one 2010 Jul 13;5(7):e11528
PloS one 2010 Jul 13;5(7):e11528
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Rat Phospho-TrkA (Y785) by Western Blot. Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line untreated (-) or treated (+) with the indicated concentrations of Recombinant Rat beta-NGF (Catalog # 556-NG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Human/Rat Phospho-TrkA (Y785) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5479), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-TrkA (Y785) at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
