LS-C152788

antibody from LSBio

Targeting: TXNRD1 GRIM-12, Trxr1, TXNR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C152788

Provider

LSBio

Product name

TRXR1 / TXNRD1 Antibody (aa137-150) LS-C152788

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Rat, Bovine, Canine, Guinea Pig, Porcine, Simian

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

WB: 40 micrograms of rat brain microsomal preparation was probed with anti-Thioredoxin Reductase 1 (1g/ml)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

WB: 40 micrograms of rat brain microsomal preparation was probed with anti-Thioredoxin Reductase 1 (1g/ml)

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ICC: CMXRos (a mitochondrial marker; 1:8000 dilution; Molecular Probes; red staining) was added to U937 cells growing in suspension, which were then incubated at 37°C for 30 min. Cells were fixed in paraformaldehyde and permeabilized. Cells were then incubated in blocking buffer (5% normal goat serum in PBS plus 0.05% Tween 20), and then in anti-TrxR1 (TrxR1; 1:100 dilution; green staining). After washing, cells were incubated with an anti-rabbit-Alexa 488 secondary antibody conjugate (1:100 dilution; Molecular Probes), washed, mounted in DAPI (blue staining) containing medium, and visualized in an Olympus IX71 confocal fluorescent microscope. Data generously provided by Ismael Samudio, Dept. of Blood and Marrow Transplantation, University of Texas M.D. Anderson Cancer Center.