Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP32538PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti Thioredoxin reductase 1 / TXNRD1 (137-150)
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated, synthetic peptide corresponding to amino acids 137-150 (CPHRIKATNNKGKEK) of Human Thioredoxin reductase 1.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoblot (Representative lot data): Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Thioredoxin Reductase 1 (1 µg/ml). Proteins were visualized using a Goat anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Thioredoxin reductase 1 (~58kDa).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunocytochemistry: CMXRos (a mitochondrial marker; 1:8,000 dilution; Molecular Probes; red staining) was added to U937 cells growing in suspension, which were then incubated at 37°C for 30 min. Cells were fixed in paraformaldehyde and permeabilized. Cells were then incubated in blocking buffer ( 5% normal goat serum in PBS plus 0.05% Tween 20), and then in anti-TrxR1 (TrxR1; 1/100 dilution, Cat.-No AP32538PU-N, green staining). After washing, cells were incubated with an anti-Rabbit-Alexa 488 secondary antibody conjugate (1/100 dilution; Molecular Probes), washed, mounted in DAPI (blue staining) containing medium, and visualized in an Olympus IX71 confocal fluorescent microscope. Data generously provided by Ismael Samudio, Dept. of Blood and Marrow Transplantation, University of Texas M.D. Anderson Cancer Center.