AP32538PU-N

antibody from Acris Antibodies GmbH

Targeting: TXNRD1 GRIM-12, Trxr1, TXNR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP32538PU-N

Provider

Acris Antibodies GmbH

Product name

anti Thioredoxin reductase 1 / TXNRD1 (137-150)

Antibody type

Polyclonal

Antigen

KLH-conjugated, synthetic peptide corresponding to amino acids 137-150 (CPHRIKATNNKGKEK) of Human Thioredoxin reductase 1.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunoblot (Representative lot data): Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Thioredoxin Reductase 1 (1 µg/ml). Proteins were visualized using a Goat anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Thioredoxin reductase 1 (~58kDa).

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunocytochemistry: CMXRos (a mitochondrial marker; 1:8,000 dilution; Molecular Probes; red staining) was added to U937 cells growing in suspension, which were then incubated at 37°C for 30 min. Cells were fixed in paraformaldehyde and permeabilized. Cells were then incubated in blocking buffer ( 5% normal goat serum in PBS plus 0.05% Tween 20), and then in anti-TrxR1 (TrxR1; 1/100 dilution, Cat.-No AP32538PU-N, green staining). After washing, cells were incubated with an anti-Rabbit-Alexa 488 secondary antibody conjugate (1/100 dilution; Molecular Probes), washed, mounted in DAPI (blue staining) containing medium, and visualized in an Olympus IX71 confocal fluorescent microscope. Data generously provided by Ismael Samudio, Dept. of Blood and Marrow Transplantation, University of Texas M.D. Anderson Cancer Center.