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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-34685 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TrxR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2. Predicted reactivity: Mouse (87%), Rat (87%), Dog (89%), Pig (89%), Rabbit (92%), Chicken (81%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Expression of TXNRD1, HSPA4L and ATP1B1 Genes Associated with the Freezability of Boar Sperm.
Mańkowska A, Gilun P, Zasiadczyk Ł, Sobiech P, Fraser L
International journal of molecular sciences 2022 Aug 18;23(16)
International journal of molecular sciences 2022 Aug 18;23(16)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TrxR1 Polyclonal Antibody detects TrxR1 protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TrxR1 protein stained by TrxR1 Polyclonal Antibody (Product # PA5-34685) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis showing protein expression of TXNRD1, HSPA4L and ATP1B1 in ( A ) the fresh pre-freeze (PF) and ( B ) frozen-thawed (FT) sperm from boars of the good and poor semen freezability (GSF and PSF, respectively) groups.
