HPA001395

antibody from Atlas Antibodies

Targeting: TXNRD1 GRIM-12, Trxr1, TXNR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA001395

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA001395, RRID:AB_1858377

Product name

Anti-TXNRD1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

SCEDGRALEGTLSELAAETDLPVVFVKQRKIGGHG
PTLKAYQEGRLQKLLKMNGPEDLPKSYDYDLIIIG
GGSGGLAAAKEAAQYGKKVMVLDFVTPTPLGTRWG
LGGTCVNVGCIPKKLMHQAALLGQALQDSRNYG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell lines PC-3 and MCF-7 using Anti-TXNRD1 antibody. Corresponding TXNRD1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human cell line A-431Lane 5: Human liver tissue

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in Leydig cells and basal cells in the seminiferous ducts.

Sample type

HUMAN