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Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- bs-5218R - Provider product page
- Provider
- Bioss
- Proper citation
- Bioss Cat#bs-5218R, RRID:AB_11111792
- Product name
- Wee1(Ser123) Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- KLH conjugated synthetic phosphopeptide derived from human Wee1 around the phosphorylation site of Ser123 [SS(p-S)PV]
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1ug/ul
- Storage
- Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
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Supportive validation
- Submitted by
- Bioss (provider)
- Main image
- Experimental details
- Lane 1: mouse embryo lysates Lane 2: mouse pancreas lysates probed with Anti Phospho-Wee1(Ser123)Polyclonal Antibody, Unconjugated (bs-5218R) at 1:200 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37°C. Predicted band 71kD. Observed band size: 71kD.
- Sample type
- Mouse
- Other comments
- Pancreas
