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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX111392 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111392, RRID:AB_2038242
- Product name
- WEE1 antibody [N3C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references A unique insertion in STARD9's motor domain regulates its stability.
Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma.
Senese S, Cheung K, Lo YC, Gholkar AA, Xia X, Wohlschlegel JA, Torres JZ
Molecular biology of the cell 2015 Feb 1;26(3):440-52
Molecular biology of the cell 2015 Feb 1;26(3):440-52
Polo-like kinase inhibitor Ro5203280 has potent antitumor activity in nasopharyngeal carcinoma.
Cheung AK, Ip JC, Lung HL, Wu JZ, Tsao SW, Lung ML
Molecular cancer therapeutics 2013 Aug;12(8):1393-401
Molecular cancer therapeutics 2013 Aug;12(8):1393-401
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WEE1 antibody [N3C2], Internal detects WEE1 protein by western blot analysis.A. 30 ?g BCL-1 whole cell lysate/extract7.5% SDS-PAGEWEE1 antibody [N3C2], Internal (GTX111392) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: H1299 7.5% SDS PAGE GTX111392 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membranes were blotted with WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:1000 and competitor's antibody (CST#13084) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WEE1 antibody [N3C2], Internal detects WEE1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: WEE1 protein stained by WEE1 antibody [N3C2], Internal (GTX111392) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CL1-0 Xenograft, using WEE1(GTX111392) antibody at 1:100 dilution.
