Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Proximity ligation assay [1]
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Validation data
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- Product number
- H00007465-D01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007465-D01P, RRID:AB_1581920
- Product name
- WEE1 purified MaxPab rabbit polyclonal antibody (D01P)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human WEE1 protein.
- Antigen sequence
MSFLSRQQPPPPRRAGAACTLRQKLIFSPCSDCEE
EEEEEEEEGSGHSTGEDSAFQEPDSPLPPARSPTE
PGPERRRSPGPAPGSPGELEEDLLLPGACPGADEA
GGGAEGDSWEEEGFGSSSPVKSPAAPYFLGSSFSP
VRCGGPGDASPRGCGARRAGEGRRSPRPDHPGTPP
HKTFRKLRLFDTPHTPKSLLSKARGIDSSSVKLRG
SSLFMDTEKSGKREFDVRQTPQVNINPFTPDSLLL
HSSGQCRRRKRTYWNDSCGEDMEASDYELEDETRP
AKRITITESNMKSRYTTEFHELEKIGSGEFGSVFK
CVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAH
AVLGQHSHVVRYFSAWAEDDHMLIQNEYRNGGSLA
DAISENYRIMSYFKEAELKDLLLQVGRGLRYIHSM
SLVHMDIKPSNIFISRTSIPNAASEEGDEDDWASN
KVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQE
NYTHLPKADIFALALTVVCAAGAEPLPRNGDQWHE
IRQGRLPRIPQVLSQEFTELLKVMIHPDPERRPSA
MALVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQ
KELKKAQMAKAAAEERALFTDRMATRSTTQSNRTS
RLIGKKMNRSVSLTIY- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of WEE1 expression in transfected 293T cell line (H00007465-T02) by WEE1 MaxPab polyclonal antibody.Lane 1: WEE1 transfected lysate(71.70 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between WEE1 and FBXW11. HeLa cells were stained with anti-WEE1 rabbit purified polyclonal 1:1200 and anti-FBXW11 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)