PA5-29303

antibody from Invitrogen Antibodies

Targeting: WEE1 WEE1A

Antibody data

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Validation data

Product number: PA5-29303 - Provider product page
Provider: Invitrogen Antibodies
Product name: WEE1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: Jurkat, Raji, NCI-H929, Neuro2A, C8D30, C2C12. Predicted reactivity: Mouse (95%), Rat (96%), Xenopus laevis (84%), Chicken (88%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.83 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

WEE1 protein structure - PA5-29303 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of WEE1 using 30 µg BCL-1 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a WEE1 polyclonal antibody (Product # PA5-29303) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of WEE1 using 30 µg of H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a WEE1 polyclonal antibody (Product # PA5-29303) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of WEE1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WEE1 Polyclonal Antibody (Product # PA5-29303) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of WEE1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a WEE1 Polyclonal Antibody (Product # PA5-29303) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using WEE1 Polyclonal Antibody (Product # PA5-29303). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with WEE1 Polyclonal Antibody (Product # PA5-29303) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of WEE1 was achieved by transfecting HeLa cells with WEE1 specific siRNA (Silencer® select Product # s21). Western blot analysis (Fig. a) was performed using whole cell extracts from the WEE1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with c-Cbl Polyclonal Antibody (Product # PA5-29303, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to WEE1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-WEE1 polyclonal Antibody (Product # PA5-29303) and a 72 kDa band corresponding to WEE1 was observed in IMR-32, BeWo, Jurkat and HeLa. Whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), BeWo (Lane 2), Jurkat (Lane 3) and HeLa (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).